BMRB Entry 11088

Title:
Solution structure of the PH domain of Dynamin-2 from human
Deposition date:
2010-01-18
Original release date:
2011-01-19
Authors:
Li, H.; Sato, M.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Li, H.; Sato, M.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PH domain of Dynamin-2 from human"  .

Assembly members:

Assembly members:
PH domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: Cell free synthesis   Host organism: E. coli - cell free   Vector: P060904-04

Data sets:
Data typeCount
13C chemical shifts494
15N chemical shifts113
1H chemical shifts789

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PH domain1

Entities:

Entity 1, PH domain 113 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYVALILEARG
2   ARGGLYTRPLEUTHRILEASNASNILESER
3   LEUMETLYSGLYGLYSERLYSGLUTYRTRP
4   PHEVALLEUTHRALAGLUSERLEUSERTRP
5   TYRLYSASPGLUGLUGLULYSGLULYSLYS
6   TYRMETLEUPROLEUASPASNLEULYSILE
7   ARGASPVALGLULYSGLYPHEMETSERASN
8   LYSHISVALPHEALAILEPHEASNTHRGLU
9   GLNARGASNVALTYRLYSASPLEUARGGLN
10   ILEGLULEUALACYSASPSERGLNGLUASP
11   VALASPSERTRPLYSALASERPHELEUARG
12   ALAGLYVAL

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.17 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB23745 BAC05190 BAC85978 BAC86481 BAC86797
GB AAA16746 AAA19736 AAA40523 AAA88025 AAD23603
REF NP_001005360 NP_001005361 NP_001005362 NP_001034609 NP_001092839
SP A6H7I5 P39052 P39054 P50570
TPG DAA27924
AlphaFold A6H7I5 P50570 P39052 P39054

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks