BMRB Entry 11086

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PDB ID: 1wyo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11086
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the CH domain of human microtubule-associated protein RP/EB family member 3

Deposition date:

2009-12-28

Original release date:

2011-01-04

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CH domain of human microtubule-associated protein RP/EB family member 3" .

Assembly members:

Assembly members:
CH domain, polymer, 159 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040621-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GSSGSSGMAVNVYSTSVTSE NLSRHDMLAWVNDSLHLNYT KIEQLCSGAAYCQFMDMLFP GCVHLRKVKFQAKLEHEYIH NFKVLQAAFKKMGVDKIIPV EKLVKGKFQDNFEFIQWFKK FFDANYDGKDYNPLLARQGQ DVAPPPNPGDQIFSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	711
15N chemical shifts	161
1H chemical shifts	1106

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CH domain	1

Entities:

Entity 1, CH domain 159 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

ALA

VAL

2

ASN

VAL

TYR

SER

THR

SER

VAL

THR

SER

GLU

3

ASN

LEU

SER

ARG

HIS

ASP

MET

LEU

ALA

TRP

4

VAL

ASN

ASP

SER

LEU

HIS

LEU

ASN

TYR

THR

5

LYS

ILE

GLU

GLN

LEU

CYS

SER

GLY

ALA

6

TYR

CYS

GLN

PHE

MET

ASP

MET

LEU

PHE

PRO

7

GLY

CYS

VAL

HIS

LEU

ARG

LYS

VAL

LYS

PHE

8

GLN

ALA

LYS

LEU

GLU

HIS

GLU

TYR

ILE

HIS

9

ASN

PHE

LYS

VAL

LEU

GLN

ALA

PHE

LYS

10

LYS

MET

GLY

VAL

ASP

LYS

ILE

PRO

VAL

11

GLU

LYS

LEU

VAL

LYS

GLY

LYS

PHE

GLN

ASP

12

ASN

PHE

GLU

PHE

ILE

GLN

TRP

PHE

LYS

13

PHE

ASP

ALA

ASN

TYR

ASP

GLY

LYS

ASP

14

TYR

ASN

PRO

LEU

ALA

ARG

GLN

GLY

GLN

15

ASP

VAL

ALA

PRO

ASN

PRO

GLY

ASP

16

GLN

ILE

PHE

SER

GLY

PRO

SER

GLY

Samples:

sample_1: CH domain, [U-100% 13C; U-100% 15N], 1.28 mM; TRIS, [U-100% 2H], 20 mM; sodium chloride 100 mM; DTT, [U-100% 2H], 1 mM; sodium azide 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N-separated NOESY	sample_1	isotropic	condition_1
3D 1H-13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WYO 3CO1 3JAK 3JAL 3JAR
DBJ	BAA82958 BAE87811 BAE88276 BAE90157 BAG35263
EMBL	CAA72060 CAG38760 CAG46643
GB	AAA96321 AAH11557 AAH57918 AAH83589 AAK07556
REF	NP_001007657 NP_001271740 NP_001289979 NP_036458 NP_579928
SP	Q5XIT1 Q6PER3 Q9UPY8
AlphaFold	Q5XIT1 Q6PER3 Q9UPY8