BMRB Entry 11085

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PDB ID: 1wyl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11085
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the CH domain of human NEDD9 interacting protein with calponin homology and LIM domains

Deposition date:

2009-12-28

Original release date:

2011-01-04

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CH domain of human NEDD9 interacting protein with calponin homology and LIM domains" .

Assembly members:

Assembly members:
CH domain, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040315-92

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GSSGSSGTQEELLRWCQEQT AGYPGVHVSDLSSSWADGLA LCALVYRLQPGLLEPSELQG LGALEATAWALKVAENELGI TPVVSAQAVVAGSDPLGLIA YLSHFHSAFKSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	472
15N chemical shifts	112
1H chemical shifts	740

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CH domain	1

Entities:

Entity 1, CH domain 116 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

GLN

GLU

2

GLU

LEU

ARG

TRP

CYS

GLN

GLU

GLN

THR

3

ALA

GLY

TYR

PRO

GLY

VAL

HIS

VAL

SER

ASP

4

LEU

SER

TRP

ALA

ASP

GLY

LEU

ALA

5

LEU

CYS

ALA

LEU

VAL

TYR

ARG

LEU

GLN

PRO

6

GLY

LEU

GLU

PRO

SER

GLU

LEU

GLN

GLY

7

LEU

GLY

ALA

LEU

GLU

ALA

THR

ALA

TRP

ALA

8

LEU

LYS

VAL

ALA

GLU

ASN

GLU

LEU

GLY

ILE

9

THR

PRO

VAL

SER

ALA

GLN

ALA

VAL

10

ALA

GLY

SER

ASP

PRO

LEU

GLY

LEU

ILE

ALA

11

TYR

LEU

SER

HIS

PHE

HIS

SER

ALA

PHE

LYS

12

SER

GLY

PRO

SER

GLY

Samples:

sample_1: CH domain, [U-100% 13C; U-100% 15N], 1.22 mM; TRIS, [U-100% 2H], 20 mM; sodium chloride 100 mM; DTT, [U-100% 2H], 1 mM; sodium azide 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N-separated NOESY	sample_1	isotropic	condition_1
3D 1H-13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WYL 2DK9
DBJ	BAB13949 BAB15124 BAB86289 BAD18727 BAH12301
EMBL	CAB59266
GB	AAH09972 AAH42144 AAH52983 AIC63549 EAW48341
REF	NP_001152763 NP_001273542 NP_073602 XP_011905848 XP_011905849
SP	Q8TDZ2
AlphaFold	Q8TDZ2