BMRB Entry 11084

Name: Solution structure of the CH domain of human calponin-2
Published: 2011-01-04

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PDB ID: 1wyn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11084
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the CH domain of human calponin-2

Deposition date:

2009-12-28

Original release date:

2011-01-04

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CH domain of human calponin-2" .

Assembly members:

Assembly members:
CH domain, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040607-10

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GSSGSSGNRLLSKYDPQKEA ELRTWIEGLTGLSIGPDFQK GLKDGTILCTLMNKLQPGSV PKINRSMQNWHQLENLSNFI KAMVSYGMNPVDLFEANDLF ESGNMTQVQVSLLALAGKAK TKGLQSGVDIGVKYSEKQER SGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	617
15N chemical shifts	151
1H chemical shifts	982

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CH domain	1

Entities:

Entity 1, CH domain 146 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

ARG

LEU

2

LEU

SER

LYS

TYR

ASP

PRO

GLN

LYS

GLU

ALA

3

GLU

LEU

ARG

THR

TRP

ILE

GLU

GLY

LEU

THR

4

GLY

LEU

SER

ILE

GLY

PRO

ASP

PHE

GLN

LYS

5

GLY

LEU

LYS

ASP

GLY

THR

ILE

LEU

CYS

THR

6

LEU

MET

ASN

LYS

LEU

GLN

PRO

GLY

SER

VAL

7

PRO

LYS

ILE

ASN

ARG

SER

MET

GLN

ASN

TRP

8

HIS

GLN

LEU

GLU

ASN

LEU

SER

ASN

PHE

ILE

9

LYS

ALA

MET

VAL

SER

TYR

GLY

MET

ASN

PRO

10

VAL

ASP

LEU

PHE

GLU

ALA

ASN

ASP

LEU

PHE

11

GLU

SER

GLY

ASN

MET

THR

GLN

VAL

GLN

VAL

12

SER

LEU

ALA

LEU

ALA

GLY

LYS

ALA

LYS

13

THR

LYS

GLY

LEU

GLN

SER

GLY

VAL

ASP

ILE

14

GLY

VAL

LYS

TYR

SER

GLU

LYS

GLN

GLU

ARG

15

SER

GLY

PRO

SER

GLY

Samples:

sample_1: CH domain, [U-100% 13C; U-100% 15N], 1.3 mM; TRIS, [U-100% 2H], 20 mM; sodium chloride 100 mM; DTT, [U-100% 2H], 1 mM; sodium azide 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N-separated NOESY	sample_1	isotropic	condition_1
3D 1H-13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WYN
GB	AAH08181 EHH29410 ERE72360
REF	XP_003813803 XP_005587402 XP_007176698 XP_007176699 XP_008068876