BMRB Entry 11083

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PDB ID: 2ell
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11083
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Leucine Rich Repeat of human Acidic leucine-rich nuclear phosphoprotein 32 family member B

Deposition date:

2009-12-16

Original release date:

2010-05-27

Authors:

Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Leucine Rich Repeat of human Acidic leucine-rich nuclear phosphoprotein 32 family member B" .

Assembly members:

Assembly members:
LRR_1 domain, polymer, 168 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: E. coli - cell free Host organism: Escherichia coli Vector: P060227-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LRR_1 domain: GSSGSSGMDMKRRIHLELRN RTPAAVRELVLDNCKSNDGK IEGLTAEFVNLEFLSLINVG LISVSNLPKLPKLKKLELSE NRIFGGLDMLAEKLPNLTHL NLSGNKLKDISTLEPLKKLE CLKSLDLFNCEVTNLNDYRE SVFKLLPQLTYLDGYDREDQ EAPDSDAE

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	739
15N chemical shifts	173
1H chemical shifts	1193

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LRR_1 domain	1

Entities:

Entity 1, LRR_1 domain 168 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

ASP

MET

2

LYS

ARG

ILE

HIS

LEU

GLU

LEU

ARG

ASN

3

ARG

THR

PRO

ALA

VAL

ARG

GLU

LEU

VAL

4

LEU

ASP

ASN

CYS

LYS

SER

ASN

ASP

GLY

LYS

5

ILE

GLU

GLY

LEU

THR

ALA

GLU

PHE

VAL

ASN

6

LEU

GLU

PHE

LEU

SER

LEU

ILE

ASN

VAL

GLY

7

LEU

ILE

SER

VAL

SER

ASN

LEU

PRO

LYS

LEU

8

PRO

LYS

LEU

LYS

LEU

GLU

LEU

SER

GLU

9

ASN

ARG

ILE

PHE

GLY

LEU

ASP

MET

LEU

10

ALA

GLU

LYS

LEU

PRO

ASN

LEU

THR

HIS

LEU

11

ASN

LEU

SER

GLY

ASN

LYS

LEU

LYS

ASP

ILE

12

SER

THR

LEU

GLU

PRO

LEU

LYS

LEU

GLU

13

CYS

LEU

LYS

SER

LEU

ASP

LEU

PHE

ASN

CYS

14

GLU

VAL

THR

ASN

LEU

ASN

ASP

TYR

ARG

GLU

15

SER

VAL

PHE

LYS

LEU

PRO

GLN

LEU

THR

16

TYR

LEU

ASP

GLY

TYR

ASP

ARG

GLU

ASP

GLN

17

GLU

ALA

PRO

ASP

SER

ASP

ALA

GLU

Samples:

sample_1: LRR_1 domain, [U-13C; U-15N], 1.3 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	2ELL 2RR6
DBJ	BAD97174 BAG36474
EMBL	CAA68855 CAA68856 CAA69265 CAH04953
GB	AAB37579 AAH13003 AAH19658 AAI02955 EAW58864
REF	NP_001030246 NP_001253837 NP_006392 XP_001156957 XP_002743164
SP	Q3SZC6 Q6A1I3 Q92688
TPG	DAA26785
AlphaFold	Q3SZC6 Q6A1I3 Q92688