BMRB Entry 11069

Title:
solution structure of 1-28 GBP (growth-blocking peptide)
Deposition date:
2009-04-07
Original release date:
2009-09-04
Authors:
Umetsu, Yoshitaka; Aizawa, Tomoyasu; Muto, Kaori; Yamamoto, Hiroko; Kamiya, Masakatsu; Kumaki, Yasuhiro; Mizuguchi, Mineyuki; Demura, Makoto; Hayakawa, Yoichi; Kawano, Keiichi
Citation:

Citation: Umetsu, Yoshitaka; Aizawa, Tomoyasu; Muto, Kaori; Yamamoto, Hiroko; Kamiya, Masakatsu; Kumaki, Yasuhiro; Mizuguchi, Mineyuki; Demura, Makoto; Hayakawa, Yoichi; Kawano, Keiichi. "C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity."  J. Biol. Chem. 284, 29625-29634 (2009).
PubMed: 19710009

Assembly members:

Assembly members:
1-28 GBP, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: armyworm   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Spodoptera not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
1-28 GBP: ENFSGGCVAGYMRTPDGRCK PTFYQLIT

Data sets:
Data typeCount
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
11-28 GBP1

Entities:

Entity 1, 1-28 GBP 28 residues - Formula weight is not available

1   GLUASNPHESERGLYGLYCYSVALALAGLY
2   TYRMETARGTHRPROASPGLYARGCYSLYS
3   PROTHRPHETYRGLNLEUILETHR

Samples:

sample_1: 1-28 GBP 3 mM; DSS 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 4.4; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • JEOL ECA 600 MHz