BMRB Entry 11048
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11048
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Masuda, Tokiha; Ito, Yutaka; Terada, Tohru; Shibata, Takehiko; Mikawa, Tsutomu. "A non-canonical DNA structure enables homologous recombination in various genetic systems." J. Biol. Chem. 284, 30230-30239 (2009).
PubMed: 19729448
Assembly members:
DNA (5'-D(*DTP*DAP*DCP*DG)-3'), polymer, 4 residues, 1231.30 Da.
ecRecT, polymer, 269 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
DNA (5'-D(*DTP*DAP*DCP*DG)-3'): TACG
ecRecT: MTKQPPIAKADLQKTQGNRA
PAAVKNSDVISFINQPSMKE
QLAAALPRHMTAERMIRIAT
TEIRKVPALGNCDTMSFVSA
IVQCSQLGLEPGSALGHAYL
LPFGNKNEKSGKKNVQLIIG
YRGMIDLARRSGQIASLSAR
VVREGDEFSFEFGLDEKLIH
RPGENEDAPVTHVYAVARLK
DGGTQFEVMTRKQIELVRSL
SKAGNNGPWVTHWEEMAKKT
AIRRLFKYLPVSIEIQRAVS
MDEKEPLTIDPADSSVLTGE
YSVIDNSEE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 37 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 | 1 |
| 2 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 2 | 1 |
| 3 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 3 | 1 |
| 4 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 4 | 1 |
| 5 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 5 | 1 |
| 6 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 6 | 1 |
| 7 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 7 | 1 |
| 8 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 8 | 1 |
| 9 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 9 | 1 |
| 10 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 10 | 1 |
| 11 | ecRecT | 2 |
Entities:
Entity 1, DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 4 residues - 1231.30 Da.
| 1 | DT | DA | DC | DG |
Entity 2, ecRecT 269 residues - Formula weight is not available
| 1 | MET | THR | LYS | GLN | PRO | PRO | ILE | ALA | LYS | ALA | ||||
| 2 | ASP | LEU | GLN | LYS | THR | GLN | GLY | ASN | ARG | ALA | ||||
| 3 | PRO | ALA | ALA | VAL | LYS | ASN | SER | ASP | VAL | ILE | ||||
| 4 | SER | PHE | ILE | ASN | GLN | PRO | SER | MET | LYS | GLU | ||||
| 5 | GLN | LEU | ALA | ALA | ALA | LEU | PRO | ARG | HIS | MET | ||||
| 6 | THR | ALA | GLU | ARG | MET | ILE | ARG | ILE | ALA | THR | ||||
| 7 | THR | GLU | ILE | ARG | LYS | VAL | PRO | ALA | LEU | GLY | ||||
| 8 | ASN | CYS | ASP | THR | MET | SER | PHE | VAL | SER | ALA | ||||
| 9 | ILE | VAL | GLN | CYS | SER | GLN | LEU | GLY | LEU | GLU | ||||
| 10 | PRO | GLY | SER | ALA | LEU | GLY | HIS | ALA | TYR | LEU | ||||
| 11 | LEU | PRO | PHE | GLY | ASN | LYS | ASN | GLU | LYS | SER | ||||
| 12 | GLY | LYS | LYS | ASN | VAL | GLN | LEU | ILE | ILE | GLY | ||||
| 13 | TYR | ARG | GLY | MET | ILE | ASP | LEU | ALA | ARG | ARG | ||||
| 14 | SER | GLY | GLN | ILE | ALA | SER | LEU | SER | ALA | ARG | ||||
| 15 | VAL | VAL | ARG | GLU | GLY | ASP | GLU | PHE | SER | PHE | ||||
| 16 | GLU | PHE | GLY | LEU | ASP | GLU | LYS | LEU | ILE | HIS | ||||
| 17 | ARG | PRO | GLY | GLU | ASN | GLU | ASP | ALA | PRO | VAL | ||||
| 18 | THR | HIS | VAL | TYR | ALA | VAL | ALA | ARG | LEU | LYS | ||||
| 19 | ASP | GLY | GLY | THR | GLN | PHE | GLU | VAL | MET | THR | ||||
| 20 | ARG | LYS | GLN | ILE | GLU | LEU | VAL | ARG | SER | LEU | ||||
| 21 | SER | LYS | ALA | GLY | ASN | ASN | GLY | PRO | TRP | VAL | ||||
| 22 | THR | HIS | TRP | GLU | GLU | MET | ALA | LYS | LYS | THR | ||||
| 23 | ALA | ILE | ARG | ARG | LEU | PHE | LYS | TYR | LEU | PRO | ||||
| 24 | VAL | SER | ILE | GLU | ILE | GLN | ARG | ALA | VAL | SER | ||||
| 25 | MET | ASP | GLU | LYS | GLU | PRO | LEU | THR | ILE | ASP | ||||
| 26 | PRO | ALA | ASP | SER | SER | VAL | LEU | THR | GLY | GLU | ||||
| 27 | TYR | SER | VAL | ILE | ASP | ASN | SER | GLU | GLU |
Samples:
sample_1: DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 mM; ecRecT 0.1 mM; NaCl 100 mM; D2O 100%
sample_conditions_1: ionic strength: 100 mM; pH: 7.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| transferred NOESY | sample_1 | isotropic | sample_conditions_1 |
| DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| TOCSY | sample_1 | isotropic | sample_conditions_1 |
| ROESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - processing
NMR spectrometers:
- Bruker Avance 600 MHz