BMRB Entry 11045
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11045
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Masuda, Tokiha; Ito, Yutaka; Terada, Tohru; Shibata, Takehiko; Mikawa, Tsutomu. "A non-canonical DNA structure enables homologous recombination in various genetic systems." J. Biol. Chem. 284, 30230-30239 (2009).
PubMed: 19729448
Assembly members:
DNA (5'-D(*DTP*DAP*DCP*DG)-3'), polymer, 4 residues, 1231.30 Da.
Mhr1p, polymer, 226 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
DNA (5'-D(*DTP*DAP*DCP*DG)-3'): TACG
Mhr1p: MKVNHSISRFRPASWFEKTK
IIPPQVYIFRNLEYGQVLYS
QFPNFSQTQVDKLFVRPNWS
NRKPSLRRDIWKCMCVVNLQ
NYKQSVHLYQNLCRLRYLRD
VAQRKESDKLRKKDSNGHVW
YSGQYRPTYCQEAVADLRES
LLKVFENATPAEKQTVPAKK
PSIYWEDPWRMGDKDKHWNY
DVFNALGLEHKLIQRVGNIA
REESVILKELAKLESHPTEQ
TEVSSQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 37 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 | 1 |
| 2 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 2 | 1 |
| 3 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 3 | 1 |
| 4 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 4 | 1 |
| 5 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 5 | 1 |
| 6 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 6 | 1 |
| 7 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 7 | 1 |
| 8 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 8 | 1 |
| 9 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 9 | 1 |
| 10 | DNA (5'-D(*DTP*DAP*DCP*DG)-3') 10 | 1 |
| 11 | Mhr1p | 2 |
Entities:
Entity 1, DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 4 residues - 1231.30 Da.
| 1 | DT | DA | DC | DG |
Entity 2, Mhr1p 226 residues - Formula weight is not available
| 1 | MET | LYS | VAL | ASN | HIS | SER | ILE | SER | ARG | PHE | ||||
| 2 | ARG | PRO | ALA | SER | TRP | PHE | GLU | LYS | THR | LYS | ||||
| 3 | ILE | ILE | PRO | PRO | GLN | VAL | TYR | ILE | PHE | ARG | ||||
| 4 | ASN | LEU | GLU | TYR | GLY | GLN | VAL | LEU | TYR | SER | ||||
| 5 | GLN | PHE | PRO | ASN | PHE | SER | GLN | THR | GLN | VAL | ||||
| 6 | ASP | LYS | LEU | PHE | VAL | ARG | PRO | ASN | TRP | SER | ||||
| 7 | ASN | ARG | LYS | PRO | SER | LEU | ARG | ARG | ASP | ILE | ||||
| 8 | TRP | LYS | CYS | MET | CYS | VAL | VAL | ASN | LEU | GLN | ||||
| 9 | ASN | TYR | LYS | GLN | SER | VAL | HIS | LEU | TYR | GLN | ||||
| 10 | ASN | LEU | CYS | ARG | LEU | ARG | TYR | LEU | ARG | ASP | ||||
| 11 | VAL | ALA | GLN | ARG | LYS | GLU | SER | ASP | LYS | LEU | ||||
| 12 | ARG | LYS | LYS | ASP | SER | ASN | GLY | HIS | VAL | TRP | ||||
| 13 | TYR | SER | GLY | GLN | TYR | ARG | PRO | THR | TYR | CYS | ||||
| 14 | GLN | GLU | ALA | VAL | ALA | ASP | LEU | ARG | GLU | SER | ||||
| 15 | LEU | LEU | LYS | VAL | PHE | GLU | ASN | ALA | THR | PRO | ||||
| 16 | ALA | GLU | LYS | GLN | THR | VAL | PRO | ALA | LYS | LYS | ||||
| 17 | PRO | SER | ILE | TYR | TRP | GLU | ASP | PRO | TRP | ARG | ||||
| 18 | MET | GLY | ASP | LYS | ASP | LYS | HIS | TRP | ASN | TYR | ||||
| 19 | ASP | VAL | PHE | ASN | ALA | LEU | GLY | LEU | GLU | HIS | ||||
| 20 | LYS | LEU | ILE | GLN | ARG | VAL | GLY | ASN | ILE | ALA | ||||
| 21 | ARG | GLU | GLU | SER | VAL | ILE | LEU | LYS | GLU | LEU | ||||
| 22 | ALA | LYS | LEU | GLU | SER | HIS | PRO | THR | GLU | GLN | ||||
| 23 | THR | GLU | VAL | SER | SER | GLN |
Samples:
sample_1: DNA (5'-D(*DTP*DAP*DCP*DG)-3') 0.5 mM; Mhr1p 0.05 mM; MgCl2 6.7 mM; NaCl 50 mM; D2O 100%
sample_conditions_1: ionic strength: 50 mM; pH: 7.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H transferred NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H ROESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - processing
NMR spectrometers:
- Bruker DRX 600 MHz