BMRB Entry 11034

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR11034
MolProbity Validation Chart

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for N-terminal DNA Recognition Domain of the Bacillus subtilis transition-state regulator SpoVT
Deposition date:
2008-03-13
Original release date:
2008-12-11
Authors:
Sullivan, Daniel; Bobay, Benjamin; Kojetin, Douglas; Thompson, Richele; Rance, Mark; Strauch, Mark; Cavanagh, John
Citation:

Citation: Sullivan, Daniel; Bobay, Benjamin; Kojetin, Douglas; Thompson, Richele; Rance, Mark; Strauch, Mark; Cavanagh, John. "Insights into the nature of DNA-binding of AbrB-like transcription factors"  Structure 16, 1702-1713 (2008).
PubMed: 19000822

Assembly members:

Assembly members:
SpoVTN, polymer, 55 residues, 6376.5 Da.

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21-b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SpoVTN: MKATGIVRRIDDLGRVVIPK EIRRTLRIREGDPLEIFVDR DGEVILKKYSPISEL

Data sets:
Data typeCount
13C chemical shifts241
15N chemical shifts49
1H chemical shifts377

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SpoVTN subunit 11
2SpoVTN subunit 21

Entities:

Entity 1, SpoVTN subunit 1 55 residues - 6376.5 Da.

1   METLYSALATHRGLYILEVALARGARGILE
2   ASPASPLEUGLYARGVALVALILEPROLYS
3   GLUILEARGARGTHRLEUARGILEARGGLU
4   GLYASPPROLEUGLUILEPHEVALASPARG
5   ASPGLYGLUVALILELEULYSLYSTYRSER
6   PROILESERGLULEU

Samples:

sample_1: SpoVTN subunit 1, [U-99% 13C; U-99% 15N], 1 – 2 mM; SpoVTN subunit 2, [U-99% 13C; U-99% 15N], 1 – 2 mM; TRIS-HCl 10 mM; potassium chloride 150 mM; EDTA 1 mM; DTT 1 mM; sodium azide 0.02%

sample_2: SpoVTN subunit 1, [U-99% 15N], 1 – 2 mM; SpoVTN subunit 2, [U-99% 15N], 1 – 2 mM; TRIS-HCl 10 mM; potassium chloride 150 mM; EDTA 1 mM; DTT 1 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 150 mM; pH: 5.8; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA05291 BAB03789 BAD42216 BAD62627 BAD74334
EMBL CAB11832 CBI41182 CCC82977 CCF03591 CCF15332
GB AAP07157 AAP24108 AAS38988 AAT29131 AAT52391
REF NP_387937 NP_829956 NP_842622 WP_000648302 WP_000648303
SP P37554
AlphaFold P37554

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks