BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11022

Title: 1H chemical shift assignment for big defensin   PubMed: 18785751

Deposition date: 2007-12-26 Original release date: 2008-11-11

Authors: Kouno, Takahide; Fujitani, Naoki; Osaki, Tsukasa; Kawabata, Shun-ichiro; Nishimura, Shin-ichiro; Mizuguchi, Mineyuki; Aizawa, Tomoyasu; Demura, Makoto; Nitta, Katsutoshi; Kawano, Keiichi

Citation: Kouno, Takahide; Fujitani, Naoki; Mizuguchi, Mineyuki; Osaki, Tsukasa; Nishimura, Shin-ichiro; Kawabata, Shun-ichiro; Aizawa, Tomoyasu; Demura, Makoto; Nitta, Katsutoshi; Kawano, Keiichi. "A novel beta-defensin structure: a potential strategy of big defensin for overcoming resistance by Gram-positive bacteria."  Biochemistry 47, 10611-10619 (2008).

Assembly members:
antimicrobial protein, polymer, 79 residues, 8649.946 Da.

Natural source:   Common Name: not available   Taxonomy ID: 6853   Superkingdom: Metazoa   Kingdom: Tachypleus   Genus/species: tridentatus not available

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
antimicrobial protein: NPLIPAIYIGATVGPSVWAY LVALVGAAAVTAANIRRASS DNHSCAGNRGWCRSKCFRHE YVDTYYSAVCGRYFCCRSR

Data sets:
Data typeCount
1H chemical shifts511

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1antimicrobial protein1

Entities:

Entity 1, antimicrobial protein 79 residues - 8649.946 Da.

1   ASNPROLEUILEPROALAILETYRILEGLY
2   ALATHRVALGLYPROSERVALTRPALATYR
3   LEUVALALALEUVALGLYALAALAALAVAL
4   THRALAALAASNILEARGARGALASERSER
5   ASPASNHISSERCYSALAGLYASNARGGLY
6   TRPCYSARGSERLYSCYSPHEARGHISGLU
7   TYRVALASPTHRTYRTYRSERALAVALCYS
8   GLYARGTYRPHECYSCYSARGSERARG

Samples:

sample_1: big defensin' 'natural abundance; .; . mM; . mM; . %; . %

sample_2: big defensin' 'natural abundance; .; . mM; . mM; . %

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP, Garrett - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAB36095
PRF 2108380A 2112271A
SP P80957