BMRB Entry 11013

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11013

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The dynein stalk head, the microtubule binding domain of dynein, derived from mouse

Deposition date:

2007-11-03

Original release date:

2008-06-18

Authors:

Shimizu, Youske; Kato, Yusuke; Morii, Hisayuki; Edamatsu, Masaki; Toyoshima, Yoko; Tanokura, Masaru

Citation:

Citation: Shimizu, Youske; Kato, Yusuke; Morii, Hisayuki; Edamatsu, Masaki; Toyoshima, Yoko; Tanokura, Masaru. "The dynein stalk head, the microtubule binding-domain of dynein: NMR assignment and ligand binding" J. Biomol. NMR 41, 89-96 (2008).
PubMed: 18491033

Assembly members:

Assembly members:
DS(8:5), polymer, 143 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET17b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DS(8:5): MEDLDKVEPAVIEAQNAVKS IKKQHLVEVRSMANPPAAVK LALESICLLLGESTTDWKQI RSIIMRENFIPTIVNFSAEE ISDAIREKMKKNYMSNPSYN YEIVNRASLACGPMVKWAIA QLNYADMLKRVEPLRNEHHH HHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list2

Data type	Count
13C chemical shifts	405
15N chemical shifts	131
1H chemical shifts	177

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 143 residues - Formula weight is not available

1

MET

GLU

ASP

LEU

ASP

LYS

VAL

GLU

PRO

ALA

2

VAL

ILE

GLU

ALA

GLN

ASN

ALA

VAL

LYS

SER

3

ILE

LYS

GLN

HIS

LEU

VAL

GLU

VAL

ARG

4

SER

MET

ALA

ASN

PRO

ALA

VAL

LYS

5

LEU

ALA

LEU

GLU

SER

ILE

CYS

LEU

6

GLY

GLU

SER

THR

ASP

TRP

LYS

GLN

ILE

7

ARG

SER

ILE

MET

ARG

GLU

ASN

PHE

ILE

8

PRO

THR

ILE

VAL

ASN

PHE

SER

ALA

GLU

9

ILE

SER

ASP

ALA

ILE

ARG

GLU

LYS

MET

LYS

10

LYS

ASN

TYR

MET

SER

ASN

PRO

SER

TYR

ASN

11

TYR

GLU

ILE

VAL

ASN

ARG

ALA

SER

LEU

ALA

12

CYS

GLY

PRO

MET

VAL

LYS

TRP

ALA

ILE

ALA

13

GLN

LEU

ASN

TYR

ALA

ASP

MET

LEU

LYS

ARG

14

VAL

GLU

PRO

LEU

ARG

ASN

GLU

HIS

15

HIS

Samples:

sample_1: DS(8:5), [U-100% 13C; U-100% 15N], 0.13 mM; potassium phosphate 10 mM; potassium chloride 75 mM; D2O 10%; H2O 90%; sodium azide 0.02%; DTT 1 mM; p-ABSF 0.1 mM; sucrose 5%

sample_conditions_1: ionic strength: 85 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, chemical shift calculation

NMR spectrometers:

Varian INOVA 600 MHz

PDB	3ERR 3J1T 3J1U 3WUQ
DBJ	BAA02996 BAA20783 BAC65531 BAE90822 BAG06711
GB	AAA41103 AAF91078 AAT74625 ACI03592 ACI03593
REF	NP_001193067 NP_001367 NP_062099 NP_084514 XP_001112455
SP	P38650 Q14204 Q9JHU4
TPG	DAA17428
AlphaFold	P38650 Q14204 Q9JHU4