BMRB Entry 11013

Title:
The dynein stalk head, the microtubule binding domain of dynein, derived from mouse
Deposition date:
2007-11-03
Original release date:
2008-06-18
Authors:
Shimizu, Youske; Kato, Yusuke; Morii, Hisayuki; Edamatsu, Masaki; Toyoshima, Yoko; Tanokura, Masaru
Citation:

Citation: Shimizu, Youske; Kato, Yusuke; Morii, Hisayuki; Edamatsu, Masaki; Toyoshima, Yoko; Tanokura, Masaru. "The dynein stalk head, the microtubule binding-domain of dynein: NMR assignment and ligand binding"  J. Biomol. NMR 41, 89-96 (2008).
PubMed: 18491033

Assembly members:

Assembly members:
DS(8:5), polymer, 143 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET17b

Data sets:
Data typeCount
13C chemical shifts405
15N chemical shifts131
1H chemical shifts177

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 143 residues - Formula weight is not available

1   METGLUASPLEUASPLYSVALGLUPROALA
2   VALILEGLUALAGLNASNALAVALLYSSER
3   ILELYSLYSGLNHISLEUVALGLUVALARG
4   SERMETALAASNPROPROALAALAVALLYS
5   LEUALALEUGLUSERILECYSLEULEULEU
6   GLYGLUSERTHRTHRASPTRPLYSGLNILE
7   ARGSERILEILEMETARGGLUASNPHEILE
8   PROTHRILEVALASNPHESERALAGLUGLU
9   ILESERASPALAILEARGGLULYSMETLYS
10   LYSASNTYRMETSERASNPROSERTYRASN
11   TYRGLUILEVALASNARGALASERLEUALA
12   CYSGLYPROMETVALLYSTRPALAILEALA
13   GLNLEUASNTYRALAASPMETLEULYSARG
14   VALGLUPROLEUARGASNGLUHISHISHIS
15   HISHISHIS

Samples:

sample_1: DS(8:5), [U-100% 13C; U-100% 15N], 0.13 mM; potassium phosphate 10 mM; potassium chloride 75 mM; D2O 10%; H2O 90%; sodium azide 0.02%; DTT 1 mM; p-ABSF 0.1 mM; sucrose 5%

sample_conditions_1: ionic strength: 85 mM; pH: 6.7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, chemical shift calculation

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA02996 BAA20783 BAC65531 BAE90822 BAG06711
GB AAA41103 AAF91078 AAT74625 ACI03592 ACI03593
REF NP_001193067 NP_001367 NP_062099 NP_084514 XP_001112455
SP P38650 Q14204 Q9JHU4
TPG DAA17428
AlphaFold Q9JHU4 P38650 Q14204

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks