BMRB Entry 11005
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11005
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Citation: Zhang, Li; Morikis, Dimitrios. "Solution structure of a bent alpha-helix" Biochemistry 46, 12959-12967 (2007).
PubMed: 17941649
Assembly members:
Bent alpha-helix, polymer, 19 residues, 1925.152 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Bent alpha-helix: XWEAQAREALAKEAQARAX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 129 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | alpha_helix | 1 |
Entities:
Entity 1, alpha_helix 19 residues - 1925.152 Da.
| 1 | ACE | TRP | GLU | ALA | GLN | ALA | ARG | GLU | ALA | LEU | ||||
| 2 | ALA | LYS | GLU | ALA | GLN | ALA | ARG | ALA | NH2 |
Samples:
sample_1: entity2 – 3 mM
sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz