BMRB Entry 10335

Name: Solution structure of the 10th filamin domain from human Filamin-B
Published: 2010-04-14

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PDB ID: 2dia
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10335
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 10th filamin domain from human Filamin-B

Deposition date:

2009-04-14

Original release date:

2010-04-14

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 10th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
Filamin domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P051205-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGPFDPSKVVASGPG LEHGKVGEAGLLSVDCSEAG PGALGLEAVSDSGTKAEVSI QNNKDGTYAVTYVPLTAGMY TLTMKYGGELVPHFPARVKV EPAVDTSSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	427
15N chemical shifts	95
1H chemical shifts	657

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 113 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

PHE

ASP

2

PRO

SER

LYS

VAL

ALA

SER

GLY

PRO

GLY

3

LEU

GLU

HIS

GLY

LYS

VAL

GLY

GLU

ALA

GLY

4

LEU

SER

VAL

ASP

CYS

SER

GLU

ALA

GLY

5

PRO

GLY

ALA

LEU

GLY

LEU

GLU

ALA

VAL

SER

6

ASP

SER

GLY

THR

LYS

ALA

GLU

VAL

SER

ILE

7

GLN

ASN

LYS

ASP

GLY

THR

TYR

ALA

VAL

8

THR

TYR

VAL

PRO

LEU

THR

ALA

GLY

MET

TYR

9

THR

LEU

THR

MET

LYS

TYR

GLY

GLU

LEU

10

VAL

PRO

HIS

PHE

PRO

ALA

ARG

VAL

LYS

VAL

11

GLU

PRO

ALA

VAL

ASP

THR

SER

GLY

PRO

12

SER

GLY

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz