BMRB Entry 10334

Name: Solution structure of the 19th filamin domain from human Filamin-B
Published: 2010-04-14

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PDB ID: 2di8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10334
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 19th filamin domain from human Filamin-B

Deposition date:

2009-04-14

Original release date:

2010-04-14

Authors:

Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 19th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
Filamin domain, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050919-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGIGDARRAKVYGRG LSEGRTFEMSDFIVDTRDAG YGGISLAVEGPSKVDIQTED LEDGTCKVSYFPTVPGVYIV STKFADEHVPGSPFTVKISG EGRVKSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	449
15N chemical shifts	98
1H chemical shifts	683

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 111 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ILE

GLY

ASP

2

ALA

ARG

ALA

LYS

VAL

TYR

GLY

ARG

GLY

3

LEU

SER

GLU

GLY

ARG

THR

PHE

GLU

MET

SER

4

ASP

PHE

ILE

VAL

ASP

THR

ARG

ASP

ALA

GLY

5

TYR

GLY

ILE

SER

LEU

ALA

VAL

GLU

GLY

6

PRO

SER

LYS

VAL

ASP

ILE

GLN

THR

GLU

ASP

7

LEU

GLU

ASP

GLY

THR

CYS

LYS

VAL

SER

TYR

8

PHE

PRO

THR

VAL

PRO

GLY

VAL

TYR

ILE

VAL

9

SER

THR

LYS

PHE

ALA

ASP

GLU

HIS

VAL

PRO

10

GLY

SER

PRO

PHE

THR

VAL

LYS

ILE

SER

GLY

11

GLU

GLY

ARG

VAL

LYS

SER

GLY

PRO

SER

12

GLY

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 0.80 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DI8
GB	AAL68448
REF	XP_006040268 XP_006040269