BMRB Entry 10333

Name: Solution structure of the filamin domain from human BK158_1 protein
Published: 2010-04-14

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PDB ID: 2di7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10333
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the filamin domain from human BK158_1 protein

Deposition date:

2009-04-14

Original release date:

2010-04-14

Authors:

Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the filamin domain from human BK158_1 protein" .

Assembly members:

Assembly members:
Filamin-type immunoglobulin domains, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050905-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin-type immunoglobulin domains: GSSGSSGETGGERQLSPEKS EIWGPGLKADVVLPARYFYI QAVDTSGNKFTSSPGEKVFQ VKVSAPEEQFTRVGVQVLDR KDGSFIVRYRMYASYKNLKV EIKFQGQHVAKSPYILKGSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	529
15N chemical shifts	109
1H chemical shifts	827

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin-type immunoglobulin domains	1

Entities:

Entity 1, Filamin-type immunoglobulin domains 124 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

THR

GLY

2

GLY

GLU

ARG

GLN

LEU

SER

PRO

GLU

LYS

SER

3

GLU

ILE

TRP

GLY

PRO

GLY

LEU

LYS

ALA

ASP

4

VAL

LEU

PRO

ALA

ARG

TYR

PHE

TYR

ILE

5

GLN

ALA

VAL

ASP

THR

SER

GLY

ASN

LYS

PHE

6

THR

SER

PRO

GLY

GLU

LYS

VAL

PHE

GLN

7

VAL

LYS

VAL

SER

ALA

PRO

GLU

GLN

PHE

8

THR

ARG

VAL

GLY

VAL

GLN

VAL

LEU

ASP

ARG

9

LYS

ASP

GLY

SER

PHE

ILE

VAL

ARG

TYR

ARG

10

MET

TYR

ALA

SER

TYR

LYS

ASN

LEU

LYS

VAL

11

GLU

ILE

LYS

PHE

GLN

GLY

GLN

HIS

VAL

ALA

12

LYS

SER

PRO

TYR

ILE

LEU

LYS

GLY

SER

GLY

13

PRO

SER

GLY

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DI7
DBJ	BAD96287
GB	AAH01297 AAQ89318 ABM82133 ABM85316 AIC57069
REF	NP_076994 XP_001094880 XP_002824457 XP_003832113 XP_003919616
SP	Q6UW63
AlphaFold	Q6UW63