BMRB Entry 10332

Name: Solution structure of the 23th Filamin domain from human Filamin C
Published: 2010-04-14

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PDB ID: 2d7q
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10332
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 23th Filamin domain from human Filamin C

Deposition date:

2009-04-14

Original release date:

2010-04-14

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the 23th Filamin domain from human Filamin C" .

Assembly members:

Assembly members:
Filamin domain, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050404-09

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGAGDPGLVSAYGPG LEGGTTGVSSEFIVNTLNAG SGALSVTIDGPSKVQLDCRE CPEGHVVTYTPMAPGNYLIA IKYGGPQHIVGSPFKAKVTG PRLSGSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	425
15N chemical shifts	95
1H chemical shifts	657

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 111 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ALA

GLY

ASP

2

PRO

GLY

LEU

VAL

SER

ALA

TYR

GLY

PRO

GLY

3

LEU

GLU

GLY

THR

GLY

VAL

SER

4

GLU

PHE

ILE

VAL

ASN

THR

LEU

ASN

ALA

GLY

5

SER

GLY

ALA

LEU

SER

VAL

THR

ILE

ASP

GLY

6

PRO

SER

LYS

VAL

GLN

LEU

ASP

CYS

ARG

GLU

7

CYS

PRO

GLU

GLY

HIS

VAL

THR

TYR

THR

8

PRO

MET

ALA

PRO

GLY

ASN

TYR

LEU

ILE

ALA

9

ILE

LYS

TYR

GLY

PRO

GLN

HIS

ILE

VAL

10

GLY

SER

PRO

PHE

LYS

ALA

LYS

VAL

THR

GLY

11

PRO

ARG

LEU

SER

GLY

SER

GLY

PRO

SER

12

GLY

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2D7Q 2NQC
EMBL	CDL67988