BMRB Entry 10326

Name: Solution structure of the 18th Filamin domain from human Filamin-B
Published: 2010-04-07

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PDB ID: 2dmc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10326
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 18th Filamin domain from human Filamin-B

Deposition date:

2009-04-02

Original release date:

2010-04-07

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 18th Filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
Filamin domain, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P051205-16

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGIPGSPFTAKITDD SRRCSQVKLGSAADFLLDIS ETDLSSLTASIKAPSGRDEP CLLKRLPNNHIGISFIPREV GEHLVSIKKNGNHVANSPVS IMVVQSEIGDSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	465
15N chemical shifts	104
1H chemical shifts	734

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 116 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ILE

PRO

GLY

2

SER

PRO

PHE

THR

ALA

LYS

ILE

THR

ASP

3

SER

ARG

CYS

SER

GLN

VAL

LYS

LEU

GLY

4

SER

ALA

ASP

PHE

LEU

ASP

ILE

SER

5

GLU

THR

ASP

LEU

SER

LEU

THR

ALA

SER

6

ILE

LYS

ALA

PRO

SER

GLY

ARG

ASP

GLU

PRO

7

CYS

LEU

LYS

ARG

LEU

PRO

ASN

HIS

8

ILE

GLY

ILE

SER

PHE

ILE

PRO

ARG

GLU

VAL

9

GLY

GLU

HIS

LEU

VAL

SER

ILE

LYS

ASN

10

GLY

ASN

HIS

VAL

ALA

ASN

SER

PRO

VAL

SER

11

ILE

MET

VAL

GLN

SER

GLU

ILE

GLY

ASP

12

SER

GLY

PRO

SER

GLY

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060324, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz