BMRB Entry 10325

Name: Solution structure of the 15th Filamin domain from human Filamin-B
Published: 2010-04-07

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PDB ID: 2dmb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10325
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 15th Filamin domain from human Filamin-B

Deposition date:

2009-04-02

Original release date:

2010-04-07

Authors:

Tomizawa, T.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 15th Filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
Filamin domain, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P051205-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGTGDASKCLATGPG IASTVKTGEEVGFVVDAKTA GKGKVTCTVLTPDGTEAEAD VIENEDGTYDIFYTAAKPGT YVIYVRFGGVDIPNSPFTVM ATDGEVTAVEEAPVNACPSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	414
15N chemical shifts	95
1H chemical shifts	635

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 124 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

GLY

ASP

2

ALA

SER

LYS

CYS

LEU

ALA

THR

GLY

PRO

GLY

3

ILE

ALA

SER

THR

VAL

LYS

THR

GLY

GLU

4

VAL

GLY

PHE

VAL

ASP

ALA

LYS

THR

ALA

5

GLY

LYS

GLY

LYS

VAL

THR

CYS

THR

VAL

LEU

6

THR

PRO

ASP

GLY

THR

GLU

ALA

GLU

ALA

ASP

7

VAL

ILE

GLU

ASN

GLU

ASP

GLY

THR

TYR

ASP

8

ILE

PHE

TYR

THR

ALA

LYS

PRO

GLY

THR

9

TYR

VAL

ILE

TYR

VAL

ARG

PHE

GLY

VAL

10

ASP

ILE

PRO

ASN

SER

PRO

PHE

THR

VAL

MET

11

ALA

THR

ASP

GLY

GLU

VAL

THR

ALA

VAL

GLU

12

GLU

ALA

PRO

VAL

ASN

ALA

CYS

PRO

SER

GLY

13

PRO

SER

GLY

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.21 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz