BMRB Entry 10324

Title:
Solution structure of the 20th Filamin domain from human Filamin-B
Deposition date:
2009-04-02
Original release date:
2010-04-07
Authors:
Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 20th Filamin domain from human Filamin-B"  .

Assembly members:

Assembly members:
Filamin domain, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Vector: P051205-17

Experimental source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Vector: P051205-17

Data sets:
Data typeCount
13C chemical shifts395
15N chemical shifts89
1H chemical shifts613

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Filamin domain1

Entities:

Entity 1, Filamin domain 102 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGALAPRO
2   SERVALALATHRVALGLYSERILECYSASP
3   LEUASNLEULYSILEPROGLUILEASNSER
4   SERASPMETSERALAHISVALTHRSERPRO
5   SERGLYARGVALTHRGLUALAGLUILEVAL
6   PROMETGLYLYSASNSERHISCYSVALARG
7   PHEVALPROGLNGLUMETGLYVALHISTHR
8   VALSERVALLYSTYRARGGLYGLNHISVAL
9   THRGLYSERPROPHEGLNPHETHRVALGLY
10   PROLEUGLYGLUGLYGLYSERGLYPROSER
11   SERGLY

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB AAL68442 AAL68443

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks