BMRB Entry 10322

Name: Solution structure of the 12th filamin domain from human Filamin-B
Published: 2010-04-07

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PDB ID: 2dic
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10322
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 12th filamin domain from human Filamin-B

Deposition date:

2009-04-02

Original release date:

2010-04-07

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 12th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
Filamin domain, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060123-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGGCQPSRVQAQGPG LKEAFTNKPNVFTVVTRGAG IGGLGITVEGPSESKINCRD NKDGSCSAEYIPFAPGDYDV NITYGGAHIPGSPFRVPVKD VVDPS

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	416
15N chemical shifts	96
1H chemical shifts	643

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 105 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

CYS

GLN

2

PRO

SER

ARG

VAL

GLN

ALA

GLN

GLY

PRO

GLY

3

LEU

LYS

GLU

ALA

PHE

THR

ASN

LYS

PRO

ASN

4

VAL

PHE

THR

VAL

THR

ARG

GLY

ALA

GLY

5

ILE

GLY

LEU

GLY

ILE

THR

VAL

GLU

GLY

6

PRO

SER

GLU

SER

LYS

ILE

ASN

CYS

ARG

ASP

7

ASN

LYS

ASP

GLY

SER

CYS

SER

ALA

GLU

TYR

8

ILE

PRO

PHE

ALA

PRO

GLY

ASP

TYR

ASP

VAL

9

ASN

ILE

THR

TYR

GLY

ALA

HIS

ILE

PRO

10

GLY

SER

PRO

PHE

ARG

VAL

PRO

VAL

LYS

ASP

11

VAL

ASP

PRO

SER

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.18 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz