BMRB Entry 10321

Name: Solution structure of the 11th filamin domain from human Filamin-B
Published: 2010-04-07

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PDB ID: 2dib
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10321
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 11th filamin domain from human Filamin-B

Deposition date:

2009-04-02

Original release date:

2010-04-07

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 11th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
Filamin domain, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P051205-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGHFPARVKVEPAVD TSRIKVFGPGIEGKDVFREA TTDFTVDSRPLTQVGGDHIK AHIANPSGASTECFVTDNAD GTYQVEYTPFEKGLHVVEVT YDDVPIPNSPFKVAVTEGCQ PSSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	507
15N chemical shifts	109
1H chemical shifts	766

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 128 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

HIS

PHE

PRO

2

ALA

ARG

VAL

LYS

VAL

GLU

PRO

ALA

VAL

ASP

3

THR

SER

ARG

ILE

LYS

VAL

PHE

GLY

PRO

GLY

4

ILE

GLU

GLY

LYS

ASP

VAL

PHE

ARG

GLU

ALA

5

THR

ASP

PHE

THR

VAL

ASP

SER

ARG

PRO

6

LEU

THR

GLN

VAL

GLY

ASP

HIS

ILE

LYS

7

ALA

HIS

ILE

ALA

ASN

PRO

SER

GLY

ALA

SER

8

THR

GLU

CYS

PHE

VAL

THR

ASP

ASN

ALA

ASP

9

GLY

THR

TYR

GLN

VAL

GLU

TYR

THR

PRO

PHE

10

GLU

LYS

GLY

LEU

HIS

VAL

GLU

VAL

THR

11

TYR

ASP

VAL

PRO

ILE

PRO

ASN

SER

PRO

12

PHE

LYS

VAL

ALA

VAL

THR

GLU

GLY

CYS

GLN

13

PRO

SER

GLY

PRO

SER

GLY

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz