BMRB Entry 10320

Name: Solution structure of the 9th filamin domain from human Filamin-B
Published: 2010-04-07

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PDB ID: 2di9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10320
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 9th filamin domain from human Filamin-B

Deposition date:

2009-04-02

Original release date:

2010-04-07

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 9th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
Filamin domain, polymer, 131 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P051205-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGDVTYDGHPVPGSP YTVEASLPPDPSKVKAHGPG LEGGLVGKPAEFTIDTKGAG TGGLGLTVEGPCEAKIECSD NGDGTCSVSYLPTKPGEYFV NILFEEVHIPGSPFKADIEM PFDPSSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	520
15N chemical shifts	107
1H chemical shifts	782

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 131 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASP

VAL

THR

2

TYR

ASP

GLY

HIS

PRO

VAL

PRO

GLY

SER

PRO

3

TYR

THR

VAL

GLU

ALA

SER

LEU

PRO

ASP

4

PRO

SER

LYS

VAL

LYS

ALA

HIS

GLY

PRO

GLY

5

LEU

GLU

GLY

LEU

VAL

GLY

LYS

PRO

ALA

6

GLU

PHE

THR

ILE

ASP

THR

LYS

GLY

ALA

GLY

7

THR

GLY

LEU

GLY

LEU

THR

VAL

GLU

GLY

8

PRO

CYS

GLU

ALA

LYS

ILE

GLU

CYS

SER

ASP

9

ASN

GLY

ASP

GLY

THR

CYS

SER

VAL

SER

TYR

10

LEU

PRO

THR

LYS

PRO

GLY

GLU

TYR

PHE

VAL

11

ASN

ILE

LEU

PHE

GLU

VAL

HIS

ILE

PRO

12

GLY

SER

PRO

PHE

LYS

ALA

ASP

ILE

GLU

MET

13

PRO

PHE

ASP

PRO

SER

GLY

PRO

SER

14

GLY

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz