BMRB Entry 10302

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PDB ID: 2dbm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10302
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the SH3 domain of human SH3-containing GRB2-like protein 2

Deposition date:

2008-12-24

Original release date:

2010-01-11

Authors:

Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the SH3 domain of human SH3-containing GRB2-like protein 2" .

Assembly members:

Assembly members:
SH3 domain, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050620-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGPCCRALYDFEPEN EGELGFKEGDIITLTNQIDE NWYEGMLHGHSGFFPINYVE ILVALPHSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	296
15N chemical shifts	67
1H chemical shifts	445

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain	1

Entities:

Entity 1, SH3 domain 73 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

CYS

2

ARG

ALA

LEU

TYR

ASP

PHE

GLU

PRO

GLU

ASN

3

GLU

GLY

GLU

LEU

GLY

PHE

LYS

GLU

GLY

ASP

4

ILE

THR

LEU

THR

ASN

GLN

ILE

ASP

GLU

5

ASN

TRP

TYR

GLU

GLY

MET

LEU

HIS

GLY

HIS

6

SER

GLY

PHE

PRO

ILE

ASN

TYR

VAL

GLU

7

ILE

LEU

VAL

ALA

LEU

PRO

HIS

SER

GLY

PRO

8

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz

PDB	2DBM
DBJ	BAC31888 BAE22364 BAE23963 BAG35315 BAG73715
EMBL	CAA67971 CAD97999 CAG46883 CAG46899
GB	AAC04764 AAC14883 AAH18385 AAI23533 AAL37407
REF	NP_001070308 NP_003017 NP_062408 NP_446387 XP_001102732
SP	O35179 Q62420 Q99962
TPG	DAA26952
AlphaFold	O35179 Q62420 Q99962