BMRB Entry 10301

Name: Solution structures of the SH3 domain of human Crk-like protein
Published: 2010-01-11

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PDB ID: 2dbk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10301
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the SH3 domain of human Crk-like protein

Deposition date:

2008-12-24

Original release date:

2010-01-11

Authors:

Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the SH3 domain of human Crk-like protein" .

Assembly members:

Assembly members:
SH3 domain, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050207-02

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGPLPSTQNGPVFAK AIQKRVPCAYDKTALALEVG DIVKVTRMNINGQWEGEVNG RKGLFPFTHVKIFDPQNPDE NESGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	327
15N chemical shifts	68
1H chemical shifts	520

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain	1

Entities:

Entity 1, SH3 domain 88 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

LEU

PRO

2

SER

THR

GLN

ASN

GLY

PRO

VAL

PHE

ALA

LYS

3

ALA

ILE

GLN

LYS

ARG

VAL

PRO

CYS

ALA

TYR

4

ASP

LYS

THR

ALA

LEU

ALA

LEU

GLU

VAL

GLY

5

ASP

ILE

VAL

LYS

VAL

THR

ARG

MET

ASN

ILE

6

ASN

GLY

GLN

TRP

GLU

GLY

GLU

VAL

ASN

GLY

7

ARG

LYS

GLY

LEU

PHE

PRO

PHE

THR

HIS

VAL

8

LYS

ILE

PHE

ASP

PRO

GLN

ASN

PRO

ASP

GLU

9

ASN

GLU

SER

GLY

PRO

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz

PDB	2BZX 2BZY 2DBK
DBJ	BAC33406 BAC34933 BAE37567
EMBL	CAA62220
GB	AAH85865 AAI31985 AAI31987 EDK97459 EDL77890
REF	NP_001008285 NP_031790 XP_002719763 XP_002926331 XP_003133042
SP	P47941 Q5U2U2
TPG	DAA20421
AlphaFold	P47941 Q5U2U2