BMRB Entry 10300

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PDB ID: 2dbj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10300
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the fn3 domain of human Proto-oncogene tyrosine-protein kinase MER precursor

Deposition date:

2008-12-24

Original release date:

2010-01-11

Authors:

Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the fn3 domain of human Proto-oncogene tyrosine-protein kinase MER precursor" .

Assembly members:

Assembly members:
Fibronectin type III domain, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050314-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fibronectin type III domain: GSSGSSGWILASTTEGAPSV APLNVTVFLNESSDNVDIRW MKPPTKQQDGELVGYRISHV WQSAGISKELLEEVGQNGSR ARISVQVHNATCTVRIAAVT RGGVGPFSDPVKIFIPAHSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	493
15N chemical shifts	118
1H chemical shifts	787

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fibronectin type III domain	1

Entities:

Entity 1, Fibronectin type III domain 124 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

TRP

ILE

LEU

2

ALA

SER

THR

GLU

GLY

ALA

PRO

SER

VAL

3

ALA

PRO

LEU

ASN

VAL

THR

VAL

PHE

LEU

ASN

4

GLU

SER

ASP

ASN

VAL

ASP

ILE

ARG

TRP

5

MET

LYS

PRO

THR

LYS

GLN

ASP

GLY

6

GLU

LEU

VAL

GLY

TYR

ARG

ILE

SER

HIS

VAL

7

TRP

GLN

SER

ALA

GLY

ILE

SER

LYS

GLU

LEU

8

LEU

GLU

VAL

GLY

GLN

ASN

GLY

SER

ARG

9

ALA

ARG

ILE

SER

VAL

GLN

VAL

HIS

ASN

ALA

10

THR

CYS

THR

VAL

ARG

ILE

ALA

VAL

THR

11

ARG

GLY

VAL

GLY

PRO

PHE

SER

ASP

PRO

12

VAL

LYS

ILE

PHE

ILE

PRO

ALA

HIS

SER

GLY

13

PRO

SER

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DBJ
REF	XP_011508793 XP_014199407