BMRB Entry 10297

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PDB ID: 2e19
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10297
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the homeobox domain from human NIL-2-A zinc finger protein, transcription factor 8

Deposition date:

2008-12-17

Original release date:

2009-12-17

Authors:

Ohnishi, S.; Tochio, N.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Ohnishi, S.; Tochio, N.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the homeobox domain from human NIL-2-A zinc finger protein, transcription factor 8" .

Assembly members:

Assembly members:
homeobox domain, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060417-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
homeobox domain: GSSGSSGQPPLKNLLSLLKA YYALNAQPSAEELSKIADSV NLPLDVVKKWFEKMQAGQIS VQSS

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	264
15N chemical shifts	62
1H chemical shifts	418

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	homeobox domain	1

Entities:

Entity 1, homeobox domain 64 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLN

PRO

2

LEU

LYS

ASN

LEU

SER

LEU

LYS

ALA

3

TYR

ALA

LEU

ASN

ALA

GLN

PRO

SER

ALA

4

GLU

LEU

SER

LYS

ILE

ALA

ASP

SER

VAL

5

ASN

LEU

PRO

LEU

ASP

VAL

LYS

TRP

6

PHE

GLU

LYS

MET

GLN

ALA

GLY

GLN

ILE

SER

7

VAL

GLN

SER

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.0 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	2E19
DBJ	BAA03259 BAA11178
GB	EMC89439 EMP31625 EOA95468 KFM04765 KFO63461
REF	NP_990462 XP_005027918 XP_005027919 XP_005027920 XP_005040695
SP	P36197
AlphaFold	P36197