BMRB Entry 10292

Title:
Solution structure of the third homeobox domain of Zinc fingers and homeoboxes protein 2
Deposition date:
2008-12-17
Original release date:
2009-12-17
Authors:
Ohnishi, S.; Sasagawa, A.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Ohnishi, S.; Sasagawa, A.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the third homeobox domain of Zinc fingers and homeoboxes protein 2"  .

Assembly members:

Assembly members:
homeobox domain, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Vector: P050404-24

Experimental source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Vector: P050404-24

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts354
15N chemical shifts78
1H chemical shifts506

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 89 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYALATYRPRO
2   ASPPHEALAPROGLNLYSPHELYSGLULYS
3   THRGLNGLYGLNVALLYSILELEUGLUASP
4   SERPHELEULYSSERSERPHEPROTHRGLN
5   ALAGLULEUASPARGLEUARGVALGLUTHR
6   LYSLEUSERARGARGGLUILEASPSERTRP
7   PHESERGLUARGARGLYSLEUARGASPSER
8   METGLUGLNALAVALLEUASPSERMETGLY
9   SERGLYLYSSERGLYPROSERSERGLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.965, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts