BMRB Entry 10290

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10290
MolProbity Validation Chart

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Title:
The solution structure of the homeobox domain of human Homeobox protein DLX-5
Deposition date:
2008-12-17
Original release date:
2009-12-17
Authors:
Sasagawa, A.; Ohnishi, S.; Tochio, N.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Sasagawa, A.; Ohnishi, S.; Tochio, N.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the homeobox domain of human Homeobox protein DLX-5"  .

Assembly members:

Assembly members:
homeobox domain, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P050509-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
homeobox domain: GSSGSSGRKPRTIYSSFQLA ALQRRFQKTQYLALPERAEL AASLGLTQTQVKIWFQNKRS KIKKSGPSSG

Data sets:
Data typeCount
13C chemical shifts280
15N chemical shifts62
1H chemical shifts433

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 70 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGLYSPRO
2   ARGTHRILETYRSERSERPHEGLNLEUALA
3   ALALEUGLNARGARGPHEGLNLYSTHRGLN
4   TYRLEUALALEUPROGLUARGALAGLULEU
5   ALAALASERLEUGLYLEUTHRGLNTHRGLN
6   VALLYSILETRPPHEGLNASNLYSARGSER
7   LYSILELYSLYSSERGLYPROSERSERGLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.35 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMNMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.933, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA06534 BAB14587 BAB69965 BAB71722 BAE34656
EMBL CAG09955 CAJ82666
GB AAA02622 AAA02623 AAA42026 AAA96145 AAB30280
REF NP_001004778 NP_001068781 NP_001084033 NP_001153132 NP_005212
SP P50575 P50577 P54655 P56178 P70396
TPG DAA30786
AlphaFold P50575 P50577 P54655 P56178 P70396

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks