BMRB Entry 10289

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PDB ID: 2da7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10289
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the homeobox domain of Zinc finger homeobox protein 1b (Smad interacting protein 1)

Deposition date:

2008-12-17

Original release date:

2009-12-17

Authors:

Ohnishi, S.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Ohnishi, S.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the homeobox domain of Zinc finger homeobox protein 1b (Smad interacting protein 1)" .

Assembly members:

Assembly members:
homeobox domain, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050404-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
homeobox domain: GSSGSSGSPINPYKDHMSVL KAYYAMNMEPNSDELLKISI AVGLPQEFVKEWFEQRKVYQ YSNSRSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	292
15N chemical shifts	63
1H chemical shifts	443

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	homeobox domain	1

Entities:

Entity 1, homeobox domain 71 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

PRO

ILE

2

ASN

PRO

TYR

LYS

ASP

HIS

MET

SER

VAL

LEU

3

LYS

ALA

TYR

ALA

MET

ASN

MET

GLU

PRO

4

ASN

SER

ASP

GLU

LEU

LYS

ILE

SER

ILE

5

ALA

VAL

GLY

LEU

PRO

GLN

GLU

PHE

VAL

LYS

6

GLU

TRP

PHE

GLU

GLN

ARG

LYS

VAL

TYR

GLN

7

TYR

SER

ASN

SER

ARG

SER

GLY

PRO

SER

8

GLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DA7
DBJ	BAA25495 BAB40819 BAC65594 BAE24572 BAG72873
EMBL	CAH90122 CAJ29798
GB	AAD56590 AAH60699 AAI27102 AAI27103 AAK52081
REF	NP_001028873 NP_001125020 NP_001165124 NP_001276450 NP_055610
SP	O60315 Q9R0G7
AlphaFold	O60315 Q9R0G7