BMRB Entry 10289

Title:
Solution structure of the homeobox domain of Zinc finger homeobox protein 1b (Smad interacting protein 1)
Deposition date:
2008-12-17
Original release date:
2009-12-17
Authors:
Ohnishi, S.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Ohnishi, S.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the homeobox domain of Zinc finger homeobox protein 1b (Smad interacting protein 1)"  .

Assembly members:

Assembly members:
homeobox domain, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P050404-18

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts292
15N chemical shifts63
1H chemical shifts443

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 71 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERPROILE
2   ASNPROTYRLYSASPHISMETSERVALLEU
3   LYSALATYRTYRALAMETASNMETGLUPRO
4   ASNSERASPGLULEULEULYSILESERILE
5   ALAVALGLYLEUPROGLNGLUPHEVALLYS
6   GLUTRPPHEGLUGLNARGLYSVALTYRGLN
7   TYRSERASNSERARGSERGLYPROSERSER
8   GLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA25495 BAB40819 BAC65594 BAE24572 BAG72873
EMBL CAH90122 CAJ29798
GB AAD56590 AAH60699 AAI27102 AAI27103 AAK52081
REF NP_001028873 NP_001125020 NP_001165124 NP_001276450 NP_055610
SP O60315 Q9R0G7
AlphaFold O60315 Q9R0G7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks