BMRB Entry 10286

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PDB ID: 2da4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10286
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the homeobox domain of the hypothetical protein, DKFZp686K21156

Deposition date:

2008-12-17

Original release date:

2009-12-17

Authors:

Ohnishi, S.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Ohnishi, S.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the homeobox domain of the hypothetical protein, DKFZp686K21156" .

Assembly members:

Assembly members:
homeobox domain, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050404-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
homeobox domain: GSSGSSGALQDRTQFSDRDL ATLKKYWDNGMTSLGSVCRE KIEAVATELNVDCEIVRTWI GNRRRKYRLMGIEVSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	319
15N chemical shifts	75
1H chemical shifts	499

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	homeobox domain	1

Entities:

Entity 1, homeobox domain 80 residues - Formula weight is not available

1	GLY	SER	SER	GLY	SER	SER	GLY	ALA	LEU	GLN
2	ASP	ARG	THR	GLN	PHE	SER	ASP	ARG	ASP	LEU
3	ALA	THR	LEU	LYS	LYS	TYR	TRP	ASP	ASN	GLY
4	MET	THR	SER	LEU	GLY	SER	VAL	CYS	ARG	GLU
5	LYS	ILE	GLU	ALA	VAL	ALA	THR	GLU	LEU	ASN
6	VAL	ASP	CYS	GLU	ILE	VAL	ARG	THR	TRP	ILE
7	GLY	ASN	ARG	ARG	ARG	LYS	TYR	ARG	LEU	MET
8	GLY	ILE	GLU	VAL	SER	GLY	PRO	SER	SER	GLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz

PDB	2DA4
REF	XP_012429181

1	GLY	SER	SER	GLY	SER	SER	GLY	ALA	LEU	GLN
2	ASP	ARG	THR	GLN	PHE	SER	ASP	ARG	ASP	LEU
3	ALA	THR	LEU	LYS	LYS	TYR	TRP	ASP	ASN	GLY
4	MET	THR	SER	LEU	GLY	SER	VAL	CYS	ARG	GLU
5	LYS	ILE	GLU	ALA	VAL	ALA	THR	GLU	LEU	ASN
6	VAL	ASP	CYS	GLU	ILE	VAL	ARG	THR	TRP	ILE
7	GLY	ASN	ARG	ARG	ARG	LYS	TYR	ARG	LEU	MET
8	GLY	ILE	GLU	VAL	SER	GLY	PRO	SER	SER	GLY

1	GLY	SER	SER	GLY	SER	SER	GLY	ALA	LEU	GLN
2	ASP	ARG	THR	GLN	PHE	SER	ASP	ARG	ASP	LEU
3	ALA	THR	LEU	LYS	LYS	TYR	TRP	ASP	ASN	GLY
4	MET	THR	SER	LEU	GLY	SER	VAL	CYS	ARG	GLU
5	LYS	ILE	GLU	ALA	VAL	ALA	THR	GLU	LEU	ASN
6	VAL	ASP	CYS	GLU	ILE	VAL	ARG	THR	TRP	ILE
7	GLY	ASN	ARG	ARG	ARG	LYS	TYR	ARG	LEU	MET
8	GLY	ILE	GLU	VAL	SER	GLY	PRO	SER	SER	GLY