BMRB Entry 10277

Name: Solution structure of the Chromo domain of chromobox homolog 2 from human
Published: 2009-12-14

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PDB ID: 2d9u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10277
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Chromo domain of chromobox homolog 2 from human

Deposition date:

2008-12-15

Original release date:

2009-12-14

Authors:

Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Chromo domain of chromobox homolog 2 from human" .

Assembly members:

Assembly members:
Chromo domain, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050613-29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Chromo domain: GSSGSSGEQVFAAECILSKR LRKGKLEYLVKWRGWSSKHN SWEPEENILDPRLLLAFQKK EHEKEVQNSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	308
15N chemical shifts	70
1H chemical shifts	492

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Chromo domain	1

Entities:

Entity 1, Chromo domain 74 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

GLN

VAL

2

PHE

ALA

GLU

CYS

ILE

LEU

SER

LYS

ARG

3

LEU

ARG

LYS

GLY

LYS

LEU

GLU

TYR

LEU

VAL

4

LYS

TRP

ARG

GLY

TRP

SER

LYS

HIS

ASN

5

SER

TRP

GLU

PRO

GLU

ASN

ILE

LEU

ASP

6

PRO

ARG

LEU

ALA

PHE

GLN

LYS

7

GLU

HIS

GLU

LYS

GLU

VAL

GLN

ASN

SER

GLY

8

PRO

SER

GLY

Samples:

sample_1: chromo domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2D9U 3H91
DBJ	BAE34242 BAG73012 BAK20222
EMBL	CAA44398 CAA73723
GB	AAH04252 AAH35199 AAI19760 AAI19761 AAI56119
REF	NP_001100541 NP_001192522 NP_005180 NP_031649 NP_116036
SP	P30658 Q14781
TPG	DAA18115
AlphaFold	P30658 Q14781