BMRB Entry 10273

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PDB ID: 2eki
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10273
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the TGS domain of human developmentally-regulated GTP-binding protein 1

Deposition date:

2008-12-15

Original release date:

2009-12-14

Authors:

Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structures of the TGS domain of human developmentally-regulated GTP-binding protein 1" .

Assembly members:

Assembly members:
TGS domain, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P051011-16

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TGS domain: GSSGSSGYLKLVRIYTKPKG QLPDYTSPVVLPYSRTTVED FCMKIHKNLIKEFKYALVWG LSVKHNPQKVGKDHTLEDED VIQIVKKSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	406
15N chemical shifts	81
1H chemical shifts	634

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TGS domain	1

Entities:

Entity 1, TGS domain 93 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

TYR

LEU

LYS

2

LEU

VAL

ARG

ILE

TYR

THR

LYS

PRO

LYS

GLY

3

GLN

LEU

PRO

ASP

TYR

THR

SER

PRO

VAL

4

LEU

PRO

TYR

SER

ARG

THR

VAL

GLU

ASP

5

PHE

CYS

MET

LYS

ILE

HIS

LYS

ASN

LEU

ILE

6

LYS

GLU

PHE

LYS

TYR

ALA

LEU

VAL

TRP

GLY

7

LEU

SER

VAL

LYS

HIS

ASN

PRO

GLN

LYS

VAL

8

GLY

LYS

ASP

HIS

THR

LEU

GLU

ASP

GLU

ASP

9

VAL

ILE

GLN

ILE

VAL

LYS

SER

GLY

PRO

10

SER

GLY

Samples:

sample_1: TGS domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9827, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz

PDB	2EKI
DBJ	BAA01555 BAC38030 BAE25890 BAE33021 BAG38025
EMBL	CAA06775 CAB61403 CAG30374 CAG46856 CAK54479
GB	AAD12240 AAH19285 AAH20803 AAH39649 AAH88410
REF	NP_001009685 NP_001029863 NP_001162241 NP_001164823 NP_001253866
SP	P32233 Q3MHP5 Q9Y295
TPG	DAA20497 DAA22636
AlphaFold	P32233 Q3MHP5 Q9Y295