BMRB Entry 10272

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10272
MolProbity Validation Chart

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Title:
Solution structures of the SH3 domain of human KIAA0418
Deposition date:
2008-12-15
Original release date:
2009-12-14
Authors:
Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the SH3 domain of human KIAA0418"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P050919-08

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGATSYMTCSAYQKV QDSEISFPAGVEVQVLEKQE SGWWYVRFGELEGWAPSHYL VLDENEQPDPSGKESGPSSG

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts76
1H chemical shifts480

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 80 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYALATHRSER
2   TYRMETTHRCYSSERALATYRGLNLYSVAL
3   GLNASPSERGLUILESERPHEPROALAGLY
4   VALGLUVALGLNVALLEUGLULYSGLNGLU
5   SERGLYTRPTRPTYRVALARGPHEGLYGLU
6   LEUGLUGLYTRPALAPROSERHISTYRLEU
7   VALLEUASPGLUASNGLUGLNPROASPPRO
8   SERGLYLYSGLUSERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9827, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

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