BMRB Entry 10269

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PDB ID: 2ee1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10269
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the Chromo domain of human chromodomain helicase-DNA-binding protein 4

Deposition date:

2008-12-15

Original release date:

2009-12-14

Authors:

Sato, M.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structures of the Chromo domain of human chromodomain helicase-DNA-binding protein 4" .

Assembly members:

Assembly members:
Chromo domain, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060904-16

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Chromo domain: GSSGSSGKPEWMMIHRILNH SVDKKGHVHYLIKWRDLPYD QASWESEDVEIQDYDLFKQS YWNH

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	285
15N chemical shifts	65
1H chemical shifts	430

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Chromo domain	1

Entities:

Entity 1, Chromo domain 64 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LYS

PRO

GLU

2

TRP

MET

ILE

HIS

ARG

ILE

LEU

ASN

HIS

3

SER

VAL

ASP

LYS

GLY

HIS

VAL

HIS

TYR

4

LEU

ILE

LYS

TRP

ARG

ASP

LEU

PRO

TYR

ASP

5

GLN

ALA

SER

TRP

GLU

SER

GLU

ASP

VAL

GLU

6

ILE

GLN

ASP

TYR

ASP

LEU

PHE

LYS

GLN

SER

7

TYR

TRP

ASN

HIS

Samples:

sample_1: Chromo domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v2.0.17, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2EE1
DBJ	BAC28749 BAD90499 BAE32198 BAG54725
EMBL	CAA60384
GB	AAH38596 AAH58578 ABZ92034 EAW88777 EAW88778
REF	NP_001193430 NP_001264 NP_001284482 NP_666091 XP_001063352
SP	Q14839 Q6PDQ2
TPG	DAA29256
AlphaFold	Q14839 Q6PDQ2