BMRB Entry 10267

Click here to enlarge.

PDB ID: 2edz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10267
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structures of the PDZ domain of mus musculus PDZ domain-containing protein 1

Deposition date:

2008-12-15

Original release date:

2009-12-14

Authors:

Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structures of the PDZ domain of mus musculus PDZ domain-containing protein 1" .

Assembly members:

Assembly members:
PDZ domain, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060313-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGMASTFNPRECKLS KQEGQNYGFFLRIEKDTDGH LIRVIEEGSPAEKAGLLDGD RVLRINGVFVDKEEHAQVVE LVRKSGNSVTLLVLDGDSYE KAVKNQVDLKELDQ

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	481
15N chemical shifts	120
1H chemical shifts	773

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain	1

Entities:

Entity 1, PDZ domain 114 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

ALA

SER

2

THR

PHE

ASN

PRO

ARG

GLU

CYS

LYS

LEU

SER

3

LYS

GLN

GLU

GLY

GLN

ASN

TYR

GLY

PHE

4

LEU

ARG

ILE

GLU

LYS

ASP

THR

ASP

GLY

HIS

5

LEU

ILE

ARG

VAL

ILE

GLU

GLY

SER

PRO

6

ALA

GLU

LYS

ALA

GLY

LEU

ASP

GLY

ASP

7

ARG

VAL

LEU

ARG

ILE

ASN

GLY

VAL

PHE

VAL

8

ASP

LYS

GLU

HIS

ALA

GLN

VAL

GLU

9

LEU

VAL

ARG

LYS

SER

GLY

ASN

SER

VAL

THR

10

LEU

VAL

LEU

ASP

GLY

ASP

SER

TYR

GLU

11

LYS

ALA

VAL

LYS

ASN

GLN

VAL

ASP

LEU

LYS

12

GLU

LEU

ASP

GLN

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v2.0.17, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz