BMRB Entry 10265

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PDB ID: 2edx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10265
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the fn3 domain of human receptor-type tyrosine-protein phosphatase F

Deposition date:

2008-12-15

Original release date:

2009-12-14

Authors:

Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the fn3 domain of human receptor-type tyrosine-protein phosphatase F" .

Assembly members:

Assembly members:
Fibronectin type III domain, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P051003-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fibronectin type III domain: GSSGSSGTIEARTAQSTPSA PPQKVMCVSMGSTTVRVSWV PPPADSRNGVITQYSVAYEA VDGEDRGRHVVDGISREHSS WDLVGLEKWTEYRVWVRAHT DVGPGPESSPVLVRTDEDVP SGPPRKVESGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	511
15N chemical shifts	122
1H chemical shifts	803

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fibronectin type III domain	1

Entities:

Entity 1, Fibronectin type III domain 134 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

ILE

GLU

2

ALA

ARG

THR

ALA

GLN

SER

THR

PRO

SER

ALA

3

PRO

GLN

LYS

VAL

MET

CYS

VAL

SER

MET

4

GLY

SER

THR

VAL

ARG

VAL

SER

TRP

VAL

5

PRO

ALA

ASP

SER

ARG

ASN

GLY

VAL

6

ILE

THR

GLN

TYR

SER

VAL

ALA

TYR

GLU

ALA

7

VAL

ASP

GLY

GLU

ASP

ARG

GLY

ARG

HIS

VAL

8

VAL

ASP

GLY

ILE

SER

ARG

GLU

HIS

SER

9

TRP

ASP

LEU

VAL

GLY

LEU

GLU

LYS

TRP

THR

10

GLU

TYR

ARG

VAL

TRP

VAL

ARG

ALA

HIS

THR

11

ASP

VAL

GLY

PRO

GLY

PRO

GLU

SER

PRO

12

VAL

LEU

VAL

ARG

THR

ASP

GLU

ASP

VAL

PRO

13

SER

GLY

PRO

ARG

LYS

VAL

GLU

SER

GLY

14

PRO

SER

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 0.7 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz