BMRB Entry 10264

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PDB ID: 1x5b
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10264
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The solution structure of the VHS domain of human Signal transducing adaptor molecule 2

Deposition date:

2008-12-03

Original release date:

2010-03-11

Authors:

Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the VHS domain of human Signal transducing adaptor molecule 2" .

Assembly members:

Assembly members:
VHS domain, polymer, 163 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P040607-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VHS domain: GSSGSSGMPLFTANPFEQDV EKATNEYNTTEDWSLIMDIC DKVGSTPNGAKDCLKAIMKR VNHKVPHVALQALTLLGACV ANCGKIFHLEVCSRDFATEV RAVIKNKAHPKVCEKLKSLM VEWSEEFQKDPQFSLISATI KSMKEEGITFPPAGSQTSGP SSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	703
15N chemical shifts	162
1H chemical shifts	1054

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VHS domain	1

Entities:

Entity 1, VHS domain 163 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

PRO

LEU

2

PHE

THR

ALA

ASN

PRO

PHE

GLU

GLN

ASP

VAL

3

GLU

LYS

ALA

THR

ASN

GLU

TYR

ASN

THR

4

GLU

ASP

TRP

SER

LEU

ILE

MET

ASP

ILE

CYS

5

ASP

LYS

VAL

GLY

SER

THR

PRO

ASN

GLY

ALA

6

LYS

ASP

CYS

LEU

LYS

ALA

ILE

MET

LYS

ARG

7

VAL

ASN

HIS

LYS

VAL

PRO

HIS

VAL

ALA

LEU

8

GLN

ALA

LEU

THR

LEU

GLY

ALA

CYS

VAL

9

ALA

ASN

CYS

GLY

LYS

ILE

PHE

HIS

LEU

GLU

10

VAL

CYS

SER

ARG

ASP

PHE

ALA

THR

GLU

VAL

11

ARG

ALA

VAL

ILE

LYS

ASN

LYS

ALA

HIS

PRO

12

LYS

VAL

CYS

GLU

LYS

LEU

LYS

SER

LEU

MET

13

VAL

GLU

TRP

SER

GLU

PHE

GLN

LYS

ASP

14

PRO

GLN

PHE

SER

LEU

ILE

SER

ALA

THR

ILE

15

LYS

SER

MET

LYS

GLU

GLY

ILE

THR

PHE

16

PRO

ALA

GLY

SER

GLN

THR

SER

GLY

PRO

17

SER

GLY

Samples:

sample_1: VHS domain, [U-13C; U-15N], 1.5 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 5 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.926, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

BMRB	18185
PDB	1X5B 2L0T
DBJ	BAF84954 BAF85536 BAG72771 BAK62690
EMBL	CAB63735 CAL38297
GB	AAC63963 AAC63964 AAH28740 AAH83912 AAI12605
REF	NP_001012085 NP_001069574 NP_001267210 NP_005834 XP_002749486
SP	O75886 Q5XHY7
TPG	DAA32691
AlphaFold	O75886 Q5XHY7