BMRB Entry 10263

Title:
Solution structures of the 6th fn3 domain of human receptor-type tyrosine-protein phosphatase F
Deposition date:
2008-11-21
Original release date:
2009-11-24
Authors:
Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the 6th fn3 domain of human receptor-type tyrosine-protein phosphatase F"  .

Assembly members:

Assembly members:
Fibronectin type III domain, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Vector: P050719-01

Experimental source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Vector: P050719-01

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts447
15N chemical shifts103
1H chemical shifts695

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fibronectin type III domain1

Entities:

Entity 1, Fibronectin type III domain 107 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROGLYARG
2   PROTHRMETMETILESERTHRTHRALAMET
3   ASNTHRALALEULEUGLNTRPHISPROPRO
4   LYSGLULEUPROGLYGLULEULEUGLYTYR
5   ARGLEUGLNTYRCYSARGALAASPGLUALA
6   ARGPROASNTHRILEASPPHEGLYLYSASP
7   ASPGLNHISPHETHRVALTHRGLYLEUHIS
8   LYSGLYTHRTHRTYRILEPHEARGLEUALA
9   ALALYSASNARGALAGLYLEUGLYGLUGLU
10   PHEGLULYSGLUILEARGTHRPROGLUASP
11   LEUSERGLYPROSERSERGLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAG54183
EMBL CAD97607

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts