BMRB Entry 10262

Name: Solution structures of the fn3 domain of human collagen alpha-1(XX) chain
Published: 2009-11-24

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PDB ID: 2dkm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10262
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the fn3 domain of human collagen alpha-1(XX) chain

Deposition date:

2008-11-21

Original release date:

2009-11-24

Authors:

Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the fn3 domain of human collagen alpha-1(XX) chain" .

Assembly members:

Assembly members:
Fibronectin type III domain, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050711-10

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fibronectin type III domain: GSSGSSGPLPPPRALTLAAV TPRTVHLTWQPSAGATHYLV RCSPASPKGEEEEREVQVGR PEVLLDGLEPGRDYEVSVQS LRGPEGSEARGIRARTPTSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	404
15N chemical shifts	86
1H chemical shifts	631

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fibronectin type III domain	1

Entities:

Entity 1, Fibronectin type III domain 104 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

LEU

PRO

2

PRO

ARG

ALA

LEU

THR

LEU

ALA

VAL

3

THR

PRO

ARG

THR

VAL

HIS

LEU

THR

TRP

GLN

4

PRO

SER

ALA

GLY

ALA

THR

HIS

TYR

LEU

VAL

5

ARG

CYS

SER

PRO

ALA

SER

PRO

LYS

GLY

GLU

6

GLU

ARG

GLU

VAL

GLN

VAL

GLY

ARG

7

PRO

GLU

VAL

LEU

ASP

GLY

LEU

GLU

PRO

8

GLY

ARG

ASP

TYR

GLU

VAL

SER

VAL

GLN

SER

9

LEU

ARG

GLY

PRO

GLU

GLY

SER

GLU

ALA

ARG

10

GLY

ILE

ARG

ALA

ARG

THR

PRO

THR

SER

GLY

11

PRO

SER

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz