BMRB Entry 10258

Name: Solution structures of the fn3 domain of human ephrin type-B receptor 1
Published: 2009-11-24

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PDB ID: 2djs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10258
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the fn3 domain of human ephrin type-B receptor 1

Deposition date:

2008-11-21

Original release date:

2009-11-24

Authors:

Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the fn3 domain of human ephrin type-B receptor 1" .

Assembly members:

Assembly members:
Fibronectin type III domain, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: Cell free synthesis Vector: P050912-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fibronectin type III domain: GSSGSSGPSTVPIMHQVSAT MRSITLSWPQPEQPNGIILD YEIRYYEKEHNEFNSSMARS QTNTARIDGLRPGMVYVVQV RARTVAGYGKFSGKMCFQTL TDSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	450
15N chemical shifts	108
1H chemical shifts	706

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fibronectin type III domain	1

Entities:

Entity 1, Fibronectin type III domain 108 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

SER

THR

2

VAL

PRO

ILE

MET

HIS

GLN

VAL

SER

ALA

THR

3

MET

ARG

SER

ILE

THR

LEU

SER

TRP

PRO

GLN

4

PRO

GLU

GLN

PRO

ASN

GLY

ILE

LEU

ASP

5

TYR

GLU

ILE

ARG

TYR

GLU

LYS

GLU

HIS

6

ASN

GLU

PHE

ASN

SER

MET

ALA

ARG

SER

7

GLN

THR

ASN

THR

ALA

ARG

ILE

ASP

GLY

LEU

8

ARG

PRO

GLY

MET

VAL

TYR

VAL

GLN

VAL

9

ARG

ALA

ARG

THR

VAL

ALA

GLY

TYR

GLY

LYS

10

PHE

SER

GLY

LYS

MET

CYS

PHE

GLN

THR

LEU

11

THR

ASP

SER

GLY

PRO

SER

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	2DJS
DBJ	BAG53024
REF	XP_004321818 XP_006883431