BMRB Entry 10257

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PDB ID: 2djr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10257
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the C2H2 type zinc finger domain of human zinc finger BED domain containing protein 2

Deposition date:

2008-11-21

Original release date:

2009-11-24

Authors:

Sato, M.; Sasagawa, A.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Sasagawa, A.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the C2H2 type zinc finger domain of human zinc finger BED domain containing protein 2" .

Assembly members:

Assembly members:
C2H2 type zinc finger domain, polymer, 76 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: Cell free synthesis Vector: P050302-53

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2H2 type zinc finger domain: GSSGSSGSEAWEYFHLAPAR AGHHPNQYATCRLCGRQVSR GPGVNVGTTALWKHLKSMHR EELEKSGHGQSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	282
15N chemical shifts	59
1H chemical shifts	423

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C2H2 type zinc finger domain	1
2	ZINC ION	2

Entities:

Entity 1, C2H2 type zinc finger domain 76 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

GLU

ALA

2

TRP

GLU

TYR

PHE

HIS

LEU

ALA

PRO

ALA

ARG

3

ALA

GLY

HIS

PRO

ASN

GLN

TYR

ALA

THR

4

CYS

ARG

LEU

CYS

GLY

ARG

GLN

VAL

SER

ARG

5

GLY

PRO

GLY

VAL

ASN

VAL

GLY

THR

ALA

6

LEU

TRP

LYS

HIS

LEU

LYS

SER

MET

HIS

ARG

7

GLU

LEU

GLU

LYS

SER

GLY

HIS

GLY

GLN

8

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: C2H2 type zinc finger domain, [U-13C; U-15N], 1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz