BMRB Entry 10254

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PDB ID: 2coc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10254
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the C-terminal PH domain of FYVE, RhoGEF and PH domain containing protein 3 (FGD3) from human

Deposition date:

2008-11-20

Original release date:

2009-11-20

Authors:

Li, H.; Tomizawa, T.; Tochio, N.; Muto, Y.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Tomizawa, T.; Tochio, N.; Muto, Y.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C-terminal PH domain of FYVE, RhoGEF and PH domain containing protein 3 (FGD3) from human" .

Assembly members:

Assembly members:
PH domain, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P050125-03

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PH domain: GSSGSSGSLLCGPLRLSESG ETWSEVWAAIPMSDPQVLHL QGGSQDGRLPRTIPLPSCKL SVPDPEERLDSGHVWKLQWA KQSWYLSASSAELQQQWLET LSTAAHSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	454
15N chemical shifts	108
1H chemical shifts	711

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PH domain	1

Entities:

Entity 1, PH domain 112 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

LEU

2

CYS

GLY

PRO

LEU

ARG

LEU

SER

GLU

SER

GLY

3

GLU

THR

TRP

SER

GLU

VAL

TRP

ALA

ILE

4

PRO

MET

SER

ASP

PRO

GLN

VAL

LEU

HIS

LEU

5

GLN

GLY

SER

GLN

ASP

GLY

ARG

LEU

PRO

6

ARG

THR

ILE

PRO

LEU

PRO

SER

CYS

LYS

LEU

7

SER

VAL

PRO

ASP

PRO

GLU

ARG

LEU

ASP

8

SER

GLY

HIS

VAL

TRP

LYS

LEU

GLN

TRP

ALA

9

LYS

GLN

SER

TRP

TYR

LEU

SER

ALA

SER

10

ALA

GLU

LEU

GLN

TRP

LEU

GLU

THR

11

LEU

SER

THR

ALA

HIS

SER

GLY

PRO

SER

12

SER

GLY

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.20 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2COC
DBJ	BAF85365 BAG63386 BAK63162
EMBL	CAH92885
GB	AAH32429 AAI11055 ACE86816 ACE87503 EAW62840
REF	NP_001077005 NP_001126690 NP_001273922 NP_149077 XP_003807897
SP	Q5JSP0 Q5R5T1
AlphaFold	Q5JSP0 Q5R5T1