BMRB Entry 10253

Name: Solution structure of the PH domain of protein kinase C, D2 type from human
Published: 2009-11-20

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PDB ID: 2coa
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10253
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the PH domain of protein kinase C, D2 type from human

Deposition date:

2008-11-20

Original release date:

2009-11-20

Authors:

Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PH domain of protein kinase C, D2 type from human" .

Assembly members:

Assembly members:
PH domain, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P041129-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PH domain: GSSGSSGTLREGWVVHYSNK DTLRKRHYWRLDCKCITLFQ NNTTNRYYKEIPLSEILTVE SAQNFSLVPPGTNPHCFEIV TANATYFVGEMPGGTPGGPS GQGAEAARGWETAIRQALMS GPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	498
15N chemical shifts	120
1H chemical shifts	802

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PH domain	1

Entities:

Entity 1, PH domain 125 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

LEU

ARG

2

GLU

GLY

TRP

VAL

HIS

TYR

SER

ASN

LYS

3

ASP

THR

LEU

ARG

LYS

ARG

HIS

TYR

TRP

ARG

4

LEU

ASP

CYS

LYS

CYS

ILE

THR

LEU

PHE

GLN

5

ASN

THR

ASN

ARG

TYR

LYS

GLU

6

ILE

PRO

LEU

SER

GLU

ILE

LEU

THR

VAL

GLU

7

SER

ALA

GLN

ASN

PHE

SER

LEU

VAL

PRO

8

GLY

THR

ASN

PRO

HIS

CYS

PHE

GLU

ILE

VAL

9

THR

ALA

ASN

ALA

THR

TYR

PHE

VAL

GLY

GLU

10

MET

PRO

GLY

THR

PRO

GLY

PRO

SER

11

GLY

GLN

GLY

ALA

GLU

ALA

ARG

GLY

TRP

12

GLU

THR

ALA

ILE

ARG

GLN

ALA

LEU

MET

SER

13

GLY

PRO

SER

GLY

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.38 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.913, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2COA
DBJ	BAC11127 BAC11508 BAG53158 BAG62084 BAG64637
EMBL	CAB43292
GB	AAI56074 AAI56947 AAK01149 EAW57441 EHH59714
REF	NP_001073349 NP_001073350 NP_001073351 NP_057541 XP_003915810
SP	Q9BZL6
AlphaFold	Q9BZL6