BMRB Entry 10252

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PDB ID: 2co9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10252
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the HMG_box domain of thymus high mobility group box protein TOX from mouse

Deposition date:

2008-11-20

Original release date:

2009-11-20

Authors:

Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the HMG_box domain of thymus high mobility group box protein TOX from mouse" .

Assembly members:

Assembly members:
HMG box, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: Cell free synthesis Vector: P040719-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HMG box: GSSGSSGKKKKKKDPNEPQK PVSAYALFFRDTQAAIKGQN PNATFGEVSKIVASMWDGLG EEQKQVYKKKTEAAKKEYLK QLAAYRASLVSKSYTDSGPS SG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	394
15N chemical shifts	90
1H chemical shifts	599

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HMG box	1

Entities:

Entity 1, HMG box 102 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LYS

2

LYS

ASP

PRO

ASN

GLU

PRO

GLN

LYS

3

PRO

VAL

SER

ALA

TYR

ALA

LEU

PHE

ARG

4

ASP

THR

GLN

ALA

ILE

LYS

GLY

GLN

ASN

5

PRO

ASN

ALA

THR

PHE

GLY

GLU

VAL

SER

LYS

6

ILE

VAL

ALA

SER

MET

TRP

ASP

GLY

LEU

GLY

7

GLU

GLN

LYS

GLN

VAL

TYR

LYS

8

THR

GLU

ALA

LYS

GLU

TYR

LEU

LYS

9

GLN

LEU

ALA

TYR

ARG

ALA

SER

LEU

VAL

10

SER

LYS

SER

TYR

THR

ASP

SER

GLY

PRO

SER

11

SER

GLY

Samples:

sample_1: HMG_box domain, [U-13C; U-15N], 1.28 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.913, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2CO9
DBJ	BAA34528 BAC26568 BAC30091 BAG11241 BAG63663
EMBL	CAF93289 CBY16474
GB	AAH16665 AAH80732 AAI47900 AAI66269 AAL78656
REF	NP_001095566 NP_001102124 NP_001121484 NP_001248418 NP_055544
SP	O94900 Q66JW3
TPG	DAA22741
AlphaFold	O94900 Q66JW3