BMRB Entry 10251

Name: Solution structure of the C-terminal PH domain of human pleckstrin 2
Published: 2009-11-20

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PDB ID: 1x1g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10251
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the C-terminal PH domain of human pleckstrin 2

Deposition date:

2008-11-20

Original release date:

2009-11-20

Authors:

Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C-terminal PH domain of human pleckstrin 2" .

Assembly members:

Assembly members:
PH domain, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: Cell free synthesis Vector: P040614-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PH domain: GSSGSSGSLSTVELSGTVVK QGYLAKQGHKRKNWKVRRFV LRKDPAFLHYYDPSKEENRP VGGFSLRGSLVSALEDNGVP TGVKGNVQGNLFKVITKDDT HYYIQASSKAERAEWIEAIK KLTSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	550
15N chemical shifts	124
1H chemical shifts	855

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PH domain	1

Entities:

Entity 1, PH domain 129 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

LEU

SER

2

THR

VAL

GLU

LEU

SER

GLY

THR

VAL

LYS

3

GLN

GLY

TYR

LEU

ALA

LYS

GLN

GLY

HIS

LYS

4

ARG

LYS

ASN

TRP

LYS

VAL

ARG

PHE

VAL

5

LEU

ARG

LYS

ASP

PRO

ALA

PHE

LEU

HIS

TYR

6

TYR

ASP

PRO

SER

LYS

GLU

ASN

ARG

PRO

7

VAL

GLY

PHE

SER

LEU

ARG

GLY

SER

LEU

8

VAL

SER

ALA

LEU

GLU

ASP

ASN

GLY

VAL

PRO

9

THR

GLY

VAL

LYS

GLY

ASN

VAL

GLN

GLY

ASN

10

LEU

PHE

LYS

VAL

ILE

THR

LYS

ASP

THR

11

HIS

TYR

ILE

GLN

ALA

SER

LYS

ALA

12

GLU

ARG

ALA

GLU

TRP

ILE

GLU

ALA

ILE

LYS

13

LYS

LEU

THR

SER

GLY

PRO

SER

GLY

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.24 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.913, Kobayashi, N. - data analysis

CYANA v1.0.7, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1X1G
REF	XP_011384462 XP_011534947 XP_011724706