BMRB Entry 10249

Name: Solution structure of the C-terminal PH domain of human pleckstrin
Published: 2009-11-20

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PDB ID: 1x05
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10249
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the C-terminal PH domain of human pleckstrin

Deposition date:

2008-11-20

Original release date:

2009-11-20

Authors:

Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C-terminal PH domain of human pleckstrin" .

Assembly members:

Assembly members:
PH domain, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: Cell free synthesis Vector: P040517-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PH domain: GSSGSSGVILKEEFRGVIIK QGCLLKQGHRRKNWKVRKFI LREDPAYLHYYDPAGAEDPL GAIHLRGCVVTSVESNSNGR KSEEENLFEIITADEVHYFL QAATPKERTEWIKAIQMASR TGKSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	556
15N chemical shifts	124
1H chemical shifts	870

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PH domain	1

Entities:

Entity 1, PH domain 129 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

VAL

ILE

LEU

2

LYS

GLU

PHE

ARG

GLY

VAL

ILE

LYS

3

GLN

GLY

CYS

LEU

LYS

GLN

GLY

HIS

ARG

4

ARG

LYS

ASN

TRP

LYS

VAL

ARG

LYS

PHE

ILE

5

LEU

ARG

GLU

ASP

PRO

ALA

TYR

LEU

HIS

TYR

6

TYR

ASP

PRO

ALA

GLY

ALA

GLU

ASP

PRO

LEU

7

GLY

ALA

ILE

HIS

LEU

ARG

GLY

CYS

VAL

8

THR

SER

VAL

GLU

SER

ASN

SER

ASN

GLY

ARG

9

LYS

SER

GLU

ASN

LEU

PHE

GLU

ILE

10

ILE

THR

ALA

ASP

GLU

VAL

HIS

TYR

PHE

LEU

11

GLN

ALA

THR

PRO

LYS

GLU

ARG

THR

GLU

12

TRP

ILE

LYS

ALA

ILE

GLN

MET

ALA

SER

ARG

13

THR

GLY

LYS

SER

GLY

PRO

SER

GLY

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.913, Kobayashi, N. - data analysis

CYANA v1.0.7, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz