BMRB Entry 10247

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PDB ID: 2co8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10247
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the LIM domain of human NEDD9 interacting protein with calponin homology and LIM domains

Deposition date:

2008-10-24

Original release date:

2009-11-03

Authors:

Sato, M.; Tomizawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Tomizawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the LIM domain of human NEDD9 interacting protein with calponin homology and LIM domains" .

Assembly members:

Assembly members:
LIM domain, polymer, 82 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P040614-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LIM domain: GSSGSSGQHQEAGAGDLCAL CGEHLYVLERLCVNGHFFHR SCFRCHTCEATLWPGGYEQH PGDGHFYCLQHLPQTDSGPS SG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	312
15N chemical shifts	73
1H chemical shifts	459

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LIM domain	1
2	ZINC ION No.1	2
3	ZINC ION No.2	2

Entities:

Entity 1, LIM domain 82 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLN

HIS

GLN

2

GLU

ALA

GLY

ALA

GLY

ASP

LEU

CYS

ALA

LEU

3

CYS

GLY

GLU

HIS

LEU

TYR

VAL

LEU

GLU

ARG

4

LEU

CYS

VAL

ASN

GLY

HIS

PHE

HIS

ARG

5

SER

CYS

PHE

ARG

CYS

HIS

THR

CYS

GLU

ALA

6

THR

LEU

TRP

PRO

GLY

TYR

GLU

GLN

HIS

7

PRO

GLY

ASP

GLY

HIS

PHE

TYR

CYS

LEU

GLN

8

HIS

LEU

PRO

GLN

THR

ASP

SER

GLY

PRO

SER

9

SER

GLY

Entity 2, ZINC ION No.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: LIM domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.01 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2CO8
DBJ	BAB13949 BAB15124 BAB86289 BAD18727 BAH12301
EMBL	CAB59266
GB	AAH09972 AAH42144 AAH52983 AIC63549 EAW48340
REF	NP_001152763 NP_001273542 NP_073602 XP_001090339 XP_001153132
SP	Q8TDZ2
AlphaFold	Q8TDZ2