BMRB Entry 10244

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PDB ID: 1x6f
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10244
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the C2H2 type zinc finger domain of human Zinc finger protein 462

Deposition date:

2008-10-24

Original release date:

2009-11-03

Authors:

Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the C2H2 type zinc finger domain of human Zinc finger protein 462" .

Assembly members:

Assembly members:
C2H2 type zinc finger domain, polymer, 88 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P041012-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2H2 type zinc finger domain: GSSGSSGLKRDFIILGNGPR LQNSTYQCKHCDSKLQSTAE LTSHLNIHNEEFQKRAKRQE RRKQLLSKQKYADGAFADFK QESGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	322
15N chemical shifts	77
1H chemical shifts	500

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C2H2 type zinc finger domain	1
2	ZINC ION	2

Entities:

Entity 1, C2H2 type zinc finger domain 88 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LEU

LYS

ARG

2

ASP

PHE

ILE

LEU

GLY

ASN

GLY

PRO

ARG

3

LEU

GLN

ASN

SER

THR

TYR

GLN

CYS

LYS

HIS

4

CYS

ASP

SER

LYS

LEU

GLN

SER

THR

ALA

GLU

5

LEU

THR

SER

HIS

LEU

ASN

ILE

HIS

ASN

GLU

6

GLU

PHE

GLN

LYS

ARG

ALA

LYS

ARG

GLN

GLU

7

ARG

LYS

GLN

LEU

SER

LYS

GLN

LYS

8

TYR

ALA

ASP

GLY

ALA

PHE

ALA

ASP

PHE

LYS

9

GLN

GLU

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: C2H2 type zinc finger domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; NTA 0.1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1X6F
DBJ	BAB47432 BAG11361
EMBL	CAH18698 CAH56168
GB	AAH36884 AAI72445 AIC60541 EAW59010 EAW59011
REF	NP_067047 XP_001109263 XP_002820132 XP_003260371 XP_003810950
SP	Q96JM2
AlphaFold	Q96JM2