BMRB Entry 10243

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PDB ID: 1x6e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10243
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of the C2H2 type zinc finger domain of human Zinc finger protein 24

Deposition date:

2008-10-24

Original release date:

2009-11-03

Authors:

Sato, M.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Sato, M.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the C2H2 type zinc finger domain of human Zinc finger protein 24" .

Assembly members:

Assembly members:
C2H2 type zinc finger domain, polymer, 72 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P040712-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2H2 type zinc finger domain: GSSGSSGIHSGEKPYGCVEC GKAFSRSSILVQHQRVHTGE KPYKCLECGKAFSQNSGLIN HQRIHTSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	268
15N chemical shifts	62
1H chemical shifts	411

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C2H2 type zinc finger domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, C2H2 type zinc finger domain 72 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ILE

HIS

SER

2

GLY

GLU

LYS

PRO

TYR

GLY

CYS

VAL

GLU

CYS

3

GLY

LYS

ALA

PHE

SER

ARG

SER

ILE

LEU

4

VAL

GLN

HIS

GLN

ARG

VAL

HIS

THR

GLY

GLU

5

LYS

PRO

TYR

LYS

CYS

LEU

GLU

CYS

GLY

LYS

6

ALA

PHE

SER

GLN

ASN

SER

GLY

LEU

ILE

ASN

7

HIS

GLN

ARG

ILE

HIS

THR

SER

GLY

PRO

SER

8

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: C2H2 type zinc finger domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 5 mM; NaN3 0.02%; ZnCl2 0.01 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz