BMRB Entry 10213

Click here to enlarge.

PDB ID: 2e29
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10213
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the GUCT domain from human ATP-dependent RNA helicase DDX50, DEAD box protein 50

Deposition date:

2008-04-09

Original release date:

2008-08-19

Authors:

Ohnishi, S.; Paakkonen, K.; Guntert, P.; Sato, M.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Ohnishi, Satoshi; Paakkonen, Kimmo; Koshiba, Seizo; Tochio, Naoya; Sato, Manami; Kobayashi, Naohiro; Harada, Takushi; Watanabe, Satoru; Muto, Yutaka; Guntert, Peter; Tanaka, Akiko; Kigawa, Takanori; Yokoyama, Shigeyuki. "Solution structure of the GUCT domain from human RNA helicase II/Gubeta reveals the RRM fold, but implausible RNA interactions." Proteins 74, 133-144 (2008).
PubMed: 18615715

Assembly members:

Assembly members:
GUCT domain, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: Cell free synthesis Vector: P060403-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GUCT domain: GSSGSSGFEPRSLITSDKGF VTMTLESLEEIQDVSCAWKE LNRKLSSNAVSQITRMCLLK GNMGVCFDVPTTESERLQAE WHDSDWILSVPA

Data sets:

assigned_chemical_shifts
- chemical_shift_1
RDCs
- RDC_list_1

Data type	Count
13C chemical shifts	387
15N chemical shifts	88
1H chemical shifts	603

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GUCT domain	1

Entities:

Entity 1, GUCT domain 92 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PHE

GLU

PRO

2

ARG

SER

LEU

ILE

THR

SER

ASP

LYS

GLY

PHE

3

VAL

THR

MET

THR

LEU

GLU

SER

LEU

GLU

4

ILE

GLN

ASP

VAL

SER

CYS

ALA

TRP

LYS

GLU

5

LEU

ASN

ARG

LYS

LEU

SER

ASN

ALA

VAL

6

SER

GLN

ILE

THR

ARG

MET

CYS

LEU

LYS

7

GLY

ASN

MET

GLY

VAL

CYS

PHE

ASP

VAL

PRO

8

THR

GLU

SER

GLU

ARG

LEU

GLN

ALA

GLU

9

TRP

HIS

ASP

SER

ASP

TRP

ILE

LEU

SER

VAL

10

PRO

ALA

Samples:

sample_1: GUCT domain, [U-13C; U-15N], 0.9 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9816, Kobayashi, N. - data analysis

CYANA v2.1, Guntert, P. - structure solution

OPALp, Koradi, R. - refinement

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2E29
DBJ	BAG57047 BAG62959
EMBL	CAB70733
GB	AAH00210 AAH00272 AAH18637 AAK29402 ABM83473
REF	NP_001245111 NP_076950 XP_002756344 XP_002820928 XP_003258245
SP	Q9BQ39
AlphaFold	Q9BQ39